Strain Data Sheet

RBRC01737

Strain Information

Image
BRC No.RBRC01737
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnmt1<tm2Enl>
Former Common nameDnmt<1c>, Dnmt<c>, Dnmt1<tm2Enl>
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells.
Lei H, Oh S P, Okano M, Juttermann R, Goss K A, Jaenisch R, Li E
Development, 122, 3195-3205 (1996). 8898232

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dnmt1DNA methyltransferase 19Dnmt1targeted mutation 2, En Li
  • autosomal dominant cerebellar ataxia, deafness and narcolepsy(MedGEN)

  • hereditary sensory neuropathy-deafness-dementia syndrome(MedGEN)
    • neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)
  • autosomal dominant cerebellar ataxia, deafness and narcolepsy(MedGEN)

  • hereditary sensory neuropathy-deafness-dementia syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal DNA methylation(MP:0008877)

  • abnormal neural tube morphology(MP:0002151)

  • decreased cell proliferation(MP:0000352)

  • embryonic growth arrest(MP:0001730)

  • embryonic lethality, complete penetrance(MP:0011092)
    • more 4 phenotypes
  • failure of initiation of embryo turning(MP:0004180)

  • failure of somite differentiation(MP:0001690)

  • impaired somite development(MP:0009768)

  • open neural tube(MP:0000929)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pgk1 polyA, mouse Dnmt1 genomic DNA
    Research application
    Specific Term and ConditionsA. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature Development 1996 122:3195-3205 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient.
    DepositorMasaki Okano (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Please use the MATERIAL TRANSFER AGREEMENT for Distribution for non-profit or for profit (English only).
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data