RBRC01737

Information1

Image
BRC No.RBRC01737
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnmt1<tm2Enl>
Former Common nameDnmt<1c>, Dnmt<c>, Dnmt1<tm2Enl>
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDevelopment, 122, 3195-3205 (1996). 8898232

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dnmt1DNA methyltransferase (cytosine-5) 19Dnmt1<tm2Enl>targeted mutation 2, En Li

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pgk1 polyA, mouse Dnmt1 genomic DNA
Research application
Specific Term and ConditionsA. A RECIPIENT must obtain a written permission of the DEPOSITOR. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature Development 1996 122:3195-3205 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient
DepositorMasaki Okano (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Please use the MATERIAL TRANSFER AGREEMENT for Distribution for non-profit or for profit (English only).

BRC mice in Publications

No Data