RBRC01733
Information1 | |
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| Image |  |
| BRC No. | RBRC01733 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Ptafr<tm1Tksh>/TkshRbrc |
| Former Common name | PAFR-KO, Platelet-activating factor (PAF) receptor knockout mouse |
| H-2 Haplotype | |
| ES Cell line | E14.1 [129P2/OlaHsd] |
| Background strain | C57BL/6NCrlCrlj |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Satoshi Ishii at the University of Tokyo in 1998. A neomycin cassette was inserted into the open reading frame of Ptafr gene. The mutant mice were backcrossed to C57BL/6. |
| Strain description | Platelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. Homozygous mutant mice showed decreased susceptibility to bacterial infection. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo. |
| Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj) |
| References | J. Exp. Med., 187, 1779-1788 (1998). 9607919 |
Health Report | |
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| Examination Date / Room / Rack | 2008/07/14Room:4-ARack:H 2008/05/20Room:4-ARack:H 2008/05/19Room:4-ARack:H 2008/03/17Room:4-ARack:H 2008/01/14Room:4-ARack:H 2007/11/12Room:4-ARack:H 2007/09/18Room:4-ARack:H 2007/07/17Room:4-ARack:H |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ptafrplatelet-activating factor receptor4Ptafr<tm1Tksh>targeted mutation 1, Takao ShimizuPAF receptor, PAFR Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pgk polyA, mouse platelet-activating factor receptor (PAF) genomic DNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 187, 1779-1788 (1998). |
| Depositor | Takao Shimizu (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Lab HP (Japanese) Genotyping protocol -PCR- |
BRC mice in Publications |
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Tanaka Y, Nagai Y, Kuroishi T, Endo Y, Sugawara S. Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock. J. Leukoc. Biol. (2011) 22131343 Kim BK, Shin EJ, Kim HC, Chung YH, Dang DK, Jung BD, Park DH, Wie MB, Kim WK, Shimizu T, Nabeshima T, Jeong JH. Platelet-activating factor receptor knockout mice are protected from MPTP-induced dopaminergic degeneration. Neurochem. Int. (2013) 23743065 |