Strain Data Sheet
RBRC01733
Information1 | |
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| Image |  |
| BRC No. | RBRC01733 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Ptafr<tm1Tksh>/TkshRbrc |
| Former Common name | PAFR-KO, Platelet-activating factor (PAF) receptor knockout mouse |
| H-2 Haplotype | |
| ES Cell line | E14.1 [129P2/OlaHsd] |
| Background strain | C57BL/6NCrlCrlj |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Satoshi Ishii at the University of Tokyo in 1998. A neomycin cassette was inserted into the open reading frame of Ptafr gene. The mutant mice were backcrossed to C57BL/6. |
| Strain description | Platelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. Homozygous mutant mice showed decreased susceptibility to bacterial infection. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo. |
| Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj) |
| References | J. Exp. Med., 187, 1779-1788 (1998). 9607919 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ptafrplatelet-activating factor receptor4Ptafr<tm1Tksh>targeted mutation 1, Takao ShimizuPAF receptor, PAFR Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pgk polyA, mouse platelet-activating factor receptor (PAF) genomic DNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 187, 1779-1788 (1998). |
| Depositor | Takao Shimizu (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Tanaka Y, Nagai Y, Kuroishi T, Endo Y, Sugawara S. Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock. J Leukoc Biol 91(3) 485-94(2012) 22131343 Kim BK, Shin EJ, Kim HC, Chung YH, Dang DK, Jung BD, Park DH, Wie MB, Kim WK, Shimizu T, Nabeshima T, Jeong JH. Platelet-activating factor receptor knockout mice are protected from MPTP-induced dopaminergic degeneration. Neurochem Int 63(3) 121-32(2013) 23743065 |