RBRC01733

Information1

Image
BRC No.RBRC01733
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ptafr<tm1Tksh>/TkshRbrc
Former Common namePAFR-KO, Platelet-activating factor (PAF) receptor knockout mouse
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Satoshi Ishii at the University of Tokyo in 1998. A neomycin cassette was inserted into the open reading frame of Ptafr gene. The mutant mice were backcrossed to C57BL/6.
Strain descriptionPlatelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. Homozygous mutant mice showed decreased susceptibility to bacterial infection. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
ReferencesJ. Exp. Med., 187, 1779-1788 (1998). 9607919

Health Report

Examination Date / Room / Rack2008/07/14Room:4-ARack:H
2008/05/20Room:4-ARack:H
2008/05/19Room:4-ARack:H
2008/03/17Room:4-ARack:H
2008/01/14Room:4-ARack:H
2007/11/12Room:4-ARack:H
2007/09/18Room:4-ARack:H
2007/07/17Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ptafrplatelet-activating factor receptor4Ptafr<tm1Tksh>targeted mutation 1, Takao Shimizu

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Pgk polyA, mouse platelet-activating factor receptor (PAF) genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 187, 1779-1788 (1998).
DepositorTakao Shimizu (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)

BRC mice in Publications

Tanaka Y, Nagai Y, Kuroishi T, Endo Y, Sugawara S.
Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock.
J. Leukoc. Biol. (2011) 22131343

Kim BK, Shin EJ, Kim HC, Chung YH, Dang DK, Jung BD, Park DH, Wie MB, Kim WK, Shimizu T, Nabeshima T, Jeong JH.
Platelet-activating factor receptor knockout mice are protected from MPTP-induced dopaminergic degeneration.
Neurochem. Int. (2013) 23743065