Strain Data Sheet

RBRC01731

Strain Information

Image
BRC No.RBRC01731
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(DRE-SEAP)/Rbrc
Former Common nameSEAP Transgenic mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Masanori Kitamura, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi.
Strain descriptionThese transgenic mice exress human secreted alkaline phoaphatase SEAP under the control of MMTV-DRE promoter.
Colony maintenance
References
Real-time detection and continuous monitoring of ER stress in vitro and in vivo by ES-TRAP: evidence for systemic, transient ER stress during endotoxemia.
Hiramatsu N, Kasai A, Hayakawa K, Yao J, Kitamura M
Nucleic Acids Res., 34, e93 (2006) 16877567

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyAUN
SEAPsecreted alkaline phosphatase (human)UNSEAPMMTV-DRE promoter [1.8 kb regulatory fragment consists of the following two parts: (1)a 484-bp fragment from the upstream region of the mouse Cyp1A1 gene that contains 4 dioxin responsive elements (DRE), and (2) a 1330-bp fragment from a part of the mouse mammary tumor virus (MMTV) promoter lacking in glucocorticoid responsive enhancers], SV40 Late mRNA poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal circulating enzyme level(MP:0001570)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMMTV-DRE promoter [1.8 kb regulatory fragment consists of the following two parts: (1)a 484-bp fragment from the upstream region of the mouse Cyp1A1 gene that contains 4 dioxin responsive elements (DRE), and (2) a 1330-bp fragment from a part of the mouse mammary tumor virus (MMTV) promoter lacking in glucocorticoid responsive enhancers], human secreted alkaline phoaphatase (SEAP) cDNA, SV40 Late mRNA poly A
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Users should use the transgenic mice under the collaboration with the establisher.
    DepositorMasanori Kitamura (University of Yamanashi)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data