Strain Data Sheet

RBRC01730

Strain Information

Image
BRC No.RBRC01730
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129S2-Ptprc<tm1Tyb>/Rbrc
Former Common nameCD45 knockout mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/cAnNCrlCrlj
Appearance
Strain developmentDeveloped by Dr. Victor L J Tybulewicz at National Institute for Medical Research in 1999. A neo was transfered into D2 ES cells to reolace the exon 12 of the Ptprc gene. BALB/c background.
Strain descriptionCD45 is a transmembrane tyrosine phosphatase implicated in the control of T cell receptor signaling. CD45 homozygous mutant mice in the BALB/c background were gorssly normal and fertile.
Colony maintenanceBackcross to BALB/c (Heterozygote x BALB/cAnN)
References
Greatly reduced efficiency of both positive and negative selection of thymocytes in CD45 tyrosine phosphatase-deficient mice.
Mee P J, Turner M, Basson M A, Costello P S, Zamoyska R, Tybulewicz V L
Eur. J. Immunol., 29, 2923-2933 (1999). 10508267

Health Report

Examination Date / Room / Rack2024/05/27Room:4-BRack:D
2024/02/26Room:4-BRack:D

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ptprcprotein tyrosine phosphatase receptor type C1Ptprctargeted mutation 1, Victor L J Tybulewicz
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)
poly AHSV TK polyA1poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal NK cell physiology(MP:0010766)

  • abnormal T cell differentiation(MP:0002145)

  • abnormal T cell physiology(MP:0002444)

  • abnormal chemokine secretion(MP:0008722)

  • abnormal cytokine secretion(MP:0003009)
    • more 12 phenotypes
  • abnormal positive T cell selection(MP:0004919)

  • decreased CD4-positive, alpha-beta T cell number(MP:0008075)

  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased double-positive T cell number(MP:0005092)

  • diarrhea(MP:0005036)

  • enlarged spleen(MP:0000691)

  • hematopoietic system phenotype(MP:0005397)

  • increased NK cell number(MP:0008044)

  • increased double-negative T cell number(MP:0005090)

  • lethality at weaning, incomplete penetrance(MP:0011084)

  • mortality/aging(MP:0010768)

  • thymus hypoplasia(MP:0001823)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase-1 (PGK) promoter, E.coli neo, HSV TK PolyA, mouse CD45 genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. immunol., 29, 2923-2933 (1999).Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Victor Tybulewicz).
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data