RBRC01730

Information1

Image
BRC No.RBRC01730
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129S2-Ptprc<tm1Tyb>/Rbrc
Former Common nameCD45 knockout mouse
H-2 Haplotype
ES Cell line
Background strainBALB/cAnNCrlCrlj
Appearance
Strain developmentDeveloped by Dr. Victor L J Tybulewicz at National Institute for Medical Research in 1999. A neo was transfered into D2 ES cells to reolace the exon 12 of the Ptprc gene. BALB/c background.
Strain descriptionCD45 is a transmembrane tyrosine phosphatase implicated in the control of T cell receptor signaling. CD45 homozygous mutant mice in the BALB/c background were gorssly normal and fertile.
Colony maintenanceBackcross to BALB/c (Heterozygote x BALB/cAnN)
ReferencesEur. J. Immunol., 29, 2923-2933 (1999). 10508267

Health Report

Examination Date / Room / Rack2015/01/19Room:4-BRack:A
2014/11/17Room:4-BRack:A
2014/09/16Room:4-BRack:A
2014/07/14Room:4-BRack:A
2014/05/19Room:4-BRack:A
2014/03/24Room:4-BRack:A
2014/01/20Room:4-BRack:A
2013/11/25Room:4-BRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ptprcprotein tyrosine phosphatase, receptor type, C1Ptprc<tm1Tyb>targeted mutation 1, Victor L J Tybulewicz

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly AHSV TK polyA1poly A

Information2

Donor DNAmouse Phosphoglycerate kinase-1 (PGK) promoter, E.coli neo, HSV TK PolyA, mouse CD45 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. immunol., 29, 2923-2933 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Victor Tybulewicz).
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data