Strain Data Sheet


Strain Information

BRC No.RBRC01726
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mfge8<tm1Osa>/OsaRbrc
Former Common nameMFG-E8 KO; MFG-E8 Knockout mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6
Strain developmentDeveloped by Drs. Rikinari Hanayama and Shigekazu Nagata at Osaka University in 2004. A neomycin cassette replaced exons 4-6 of MFGE-8 gene. The mutant mice were backcrossed to C57BL/6N for at least 9 times.
Strain descriptionB6;129-Mfge8<tm1Osa>. Apoptotic cells are rapidly uptaken by macrophages. This process is essential to prevent inflammation and the autoimmune response against intracellular antigens released from dying cells. Milk fat globule epidermal growth factor 8 (MFG-E8) is a protein that specifically binds to apoptotic cells and enhance the uptake of apoptotic cells by macrophages. The MFG-E8 deficient mice developed splenomegaly and suffered from glomerulonephritis as a result of autoantibody production. MFG-E8 is considered to have a critical role in removing apoptotic B cells in spleen and lymph nodes, and its deficiency can lead to autoimmune disease.
Colony maintenanceHomozygote x Homozygote
ReferencesScience, 304, 1147-1150 (2004). 15155946

Health Report

Examination Date / Room / Rack2024/04/08Room:3-BRack:A


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mfge8milk fat globule-EGF factor 8 protein7Mfge8<tm1Osa>targeted mutation 1, Shigekazu NagataMFG-E8, Mfgm, SED1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase-1 promoter, E. coli neo, mouse MFG-E8 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 304, 1147-1150 (2004).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
DepositorShigekazu Nagata (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-
Mouse of the Month Nov 2006

BRC mice in Publications

Chiang HY, Chu PH, Chen SC, Lee TH.
MFG-E8 promotes osteogenic transdifferentiation of smooth muscle cells and vascular calcification by regulating TGF-β1 signaling.
Commun Biol 5(1) 364(2022) 35440618