RBRC01726

Information1

Image
BRC No.RBRC01726
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Mfge8<tm1Osa>/OsaRbrc
Former Common nameMFG-E8 KO; MFG-E8 Knockout mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Drs. Rikinari Hanayama and Shigekazu Nagata at Osaka University in 2004. A neomycin cassette replaced exons 4-6 of MFGE-8 gene. The mutant mice were backcrossed to C57BL/6N for at least 9 times.
Strain descriptionB6;129-Mfge8<tm1Osa>. Apoptotic cells are rapidly uptaken by macrophages. This process is essential to prevent inflammation and the autoimmune response against intracellular antigens released from dying cells. Milk fat globule epidermal growth factor 8 (MFG-E8) is a protein that specifically binds to apoptotic cells and enhance the uptake of apoptotic cells by macrophages. The MFG-E8 deficient mice developed splenomegaly and suffered from glomerulonephritis as a result of autoantibody production. MFG-E8 is considered to have a critical role in removing apoptotic B cells in spleen and lymph nodes, and its deficiency can lead to autoimmune disease.
Colony maintenanceBackcross to C57BL/6(Heterozygote x C57BL/6NCrlj)
ReferencesScience, 304, 1147-1150 (2004). 15155946

Health Report

Examination Date / Room / Rack2020/06/01Room:3-BRack:A
2020/02/17Room:3-BRack:A
2019/12/16Room:3-BRack:A
2019/10/21Room:3-BRack:A
2019/08/26Room:3-BRack:A
2019/06/17Room:3-BRack:A
2019/04/15Room:3-BRack:A
2018/12/17Room:3-BRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mfge8milk fat globule-EGF factor 8 protein7Mfge8<tm1Osa>targeted mutation 1, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase-1 promoter, E. coli neo, mouse MFG-E8 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 304, 1147-1150 (2004).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
DepositorShigekazu Nagata (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Lab HP
Genotyping protocol -PCR-
Mouse of the Month Nov 2006

BRC mice in Publications

No Data