BRC No.RBRC01725
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Dnase2a<tm1Osa>/OsaRbrc
Former Common nameDNase II Hetero; DNase II Hetero Knockout mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Kohki Kawane and Shigekazu Nagata at Osaka University in 2001. A neomycin cassette replaced exons 1-5 and part of 6 of the Dnase2a gene. The mutant mice were backcrossed to C57BL/6.
Strain descriptionB6;129-Dnase2a<tm1Osa>. In macrophage, DNase II, deoxyribonuclease II is responsible for digesting nuclear DNA expelled from erythroid precuursor cells. Homozygous null mice for DNase II are embryonic lethal due to sever anemia, and impaired definitive erythropoiesis in the fetal liver. This strain is useful for elucidation of the function of DNase II gene.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
ReferencesScience, 292, 1546-1549 (2001). 11375492

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dnase2adeoxyribonuclease II alpha8Dnase2a<tm1Osa>targeted mutation 1, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Dnase2a genomic DNA, Phage P1 loxP site [update May 21, 2015]
Research applicationMouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 292, 1546-1549 (2001).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
DepositorShigekazu Nagata (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Chan MP, Onji M, Fukui R, Kawane K, Shibata T, Saitoh S, Ohto U, Shimizu T, Barber GN, Miyake K.
DNase II-dependent DNA digestion is required for DNA sensing by TLR9.
Nat Commun 6 5853(2015) 25600358

Ahn J, Gutman D, Saijo S, Barber GN.
STING manifests self DNA-dependent inflammatory disease.
Proc. Natl. Acad. Sci. U.S.A. (2012) 23132945