Strain Data Sheet


Strain Information

BRC No.RBRC01723
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Dffb<tm1Osa>/OsaRbrc
Former Common nameCAD KO; CAD Knockout mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Kohki Kawane and Shigekazu Nagata at Osaka University in 2003. A neomycin cassette replaced exons 1-6 and part of 7 of Dffb gene. The mutant mice were backcrossed to C57BL/6.
Strain descriptionB6;129-Dffb<tm1Osa>. CAD, Caspase-activated DNase is an endonuclease that is activated in apoptotic cells. CAD deficient thymocytes did not undergo apoptotic DNA fragmentation, but their DNA was digested by thymic macrophages that effectively phagocytosed the apoptotic thymocytes. This strain is useful for elucidation of the function of CAD gene.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj) Homozygous mutant mice show no gross developmental abnormalities and are fertile with normal fecundity.
ReferencesNat. Immunol., 4, 138-144 (2003). 12524536

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DffbDNA fragmentation factor, beta subunit4Dffb<tm1Osa>targeted mutation 1, Shigekazu Nagata40kDa, 5730477D02Rik, caspase-activated DNase, CPAN, DFF40, Didff

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Dffb (CAD) genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. immunol., 4, 138-144 (2003).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Gao L, Huang K, Jiang DS, Liu X, Huang D, Li H, Zhang XD, Huang K.
Novel role for caspase-activated DNase in the regulation of pathological cardiac hypertrophy.
Hypertension 65(4) 871-81(2015) 25646292