Strain Data Sheet

RBRC01721

Strain Information

Image
BRC No.RBRC01721
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Emu-BCL2L1)87Nnz/Rbrc
Former Common nameBcl-xL Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Gabriel Nunez at University of Michigan Medical School in 1995. The human Bcl-xL under the control of the SV40 promoter and IgH enhancer (SV40-EH casette) was injected into the pronuclei of (C57BL/6 x SJL/J)F2 fertilized egges.
Strain descriptionC57BL/6-Tg(SV40-BCL2L1)87Nnz. Bcl-xL, a member of the bcl-2 amily, is expressed in pre-B cells but downregulated at the immature and mature stages of B cell development. This transgenic mouse strain expresses the human Bcl-xL gene within B cell lineage under the control of the SV40 promoter and B IgH enhancer. The transgenic mice show marked accumulation of peripheral B cells in lymphoid organs and enhanced B cell survival in vitro.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6NCrj).
References
bcl-x exhibits regulated expression during B cell development and activation and modulates lymphocyte survival in transgenic mice.
Grillot D A, Merino R, Pena J C, Fanslow W C, Finkelman F D, Thompson C B, Nunez G
J. Exp. Med., 183, 381-391 (1996). 8627151

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
BCL2L1Bcl2-like 1UNBCL2L1mouse Immunogloblin Eu enhancer, SV 40 early promoter, SV40 poly A additional signal
poly ASV40 polyA signal sequenceUNpoly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal peripheral B cell anergy(MP:0008746)

  • decreased B cell apoptosis(MP:0008783)

  • increased B cell proliferation(MP:0005154)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse IgH enhancer, human Bcl-xL cDNA, SV40 early promoter and poly A (SV40-EH cassette)
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 183, 381-391 (1991).Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Timothy W. Behens).
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data