RBRC01721

Information1

Image
BRC No.RBRC01721
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameBcl-xL Tg
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Gabriel Nunez at University of Michigan Medical School in 1995. The human Bcl-xL under the control of the SV40 promoter and IgH enhancer (SV40-EH casette) was injected into the pronuclei of (C57BL/6 x SJL/J)F2 fertilized egges.
Strain descriptionC57BL/6-Tg(SV40-BCL2L1)87Nnz. Bcl-xL, a member of the bcl-2 amily, is expressed in pre-B cells but downregulated at the immature and mature stages of B cell development. This transgenic mouse strain expresses the human Bcl-xL gene within B cell lineage under the control of the SV40 promoter and B IgH enhancer. The transgenic mice show marked accumulation of peripheral B cells in lymphoid organs and enhanced B cell survival in vitro.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6NCrj).
ReferencesJ. Exp. Med., 183, 381-391 (1996). 8627151

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
BCL2L1Bcl2-like 1UNBCL2L1mouse Immunogloblin Eu enhancer, SV 40 early promoter, SV40 poly A additional signal

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ASV40 polyA signal sequenceUNpoly A

Information2

Donor DNAmouse IgH enhancer, human Bcl-xL cDNA, SV40 early promoter and poly A (SV40-EH cassete)
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 183, 381-391 (1991).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Timothy W. Behens).
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data