Strain Data Sheet

RBRC01712

Strain Information

Image
BRC No.RBRC01712
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameD2.129S-Ahr<tm1Yfk>
Former Common nameAhR(DBA/2)+/- mouse
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Drs. Yoshiaki Fujii-Kuriyama and Junsei Mimura at Tohoku University in 1997. The mutant mice are backcrossed to DBA/2.
Strain descriptionThe aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediate to catalyze xenobiotic Phase I metabolism.
Colony maintenanceBackcross to DBA/2 (Heterozygote x DBA/2JJcl)
References
Loss of teratogenic response to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice lacking the Ah (dioxin) receptor.
Mimura J, Yamashita K, Nakamura K, Morita M, Takagi T N, Nakao K, Ema M, Sogawa K, Yasuda M, Katsuki M, Fujii-Kuriyama Y
Genes Cells, 2, 645-654 (1997). 9427285

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ahraryl-hydrocarbon receptor12Ahrtargeted mutation 1, Yoshiaki Fujii-Kuriyama
  • retinitis pigmentosa 85(MedGEN)
    • lacZbeta-galactosidase (E. coli)12
  • retinitis pigmentosa 85(MedGEN)
    • neoneomycin resistance gene (E. coli)12herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • retinitis pigmentosa 85(MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)12nls
  • retinitis pigmentosa 85(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal horizontal vestibuloocular reflex(MP:0011042)

  • abnormal optokinetic reflex(MP:0009619)

  • abnormal urinary bladder morphology(MP:0000538)

  • abnormal urinary bladder urothelium morphology(MP:0000540)

  • abnormal urinary bladder vasculature morphology(MP:0011773)
    • more 13 phenotypes
  • abnormal xenobiotic pharmacokinetics(MP:0008875)

  • cystolithiasis(MP:0011625)

  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • disorganized retina layers(MP:0001328)

  • homeostasis/metabolism phenotype(MP:0005376)

  • increased urinary bladder carcinoma incidence(MP:0009550)

  • increased urine uric acid level(MP:0009810)

  • nervous system phenotype(MP:0003631)

  • postnatal growth retardation(MP:0001732)

  • renal/urinary system phenotype(MP:0005367)

  • skeleton phenotype(MP:0005390)

  • urinary bladder fibrosis(MP:0011769)

  • urinary bladder inflammation(MP:0009646)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ, Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli neo, mouse Ahr genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), SV40 T antigen intron and polyadenylation signal
    Research applicationMetabolism Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYoshiaki Fujii (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ishikawa T, Okinaga H, Takahashi S, Numakura M, Mashimo Y, Yoshimura N, Maeda T, Inoue D, Okazaki R, Kinoshita M, Jameson JL, Teramoto T.
    Serum from methimazole-treated patients induces activation of aryl hydrocarbon receptor, a transcription factor that binds to dioxin-response elements.
    Thyroid 22(8) 769-77(2012) 22784254