Strain Data Sheet
RBRC01710
Information1 | |
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| Image |  |
| BRC No. | RBRC01710 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129S-Ahr<tm1Yfk> |
| Former Common name | AhR(C57BL6j)-/- mouse |
| H-2 Haplotype | |
| ES Cell line | CCE/EK.CCE [129S/SvEv-Gpi1<c>] |
| Background strain | |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Drs. Yoshiaki Fujii-Kuriyama and Junsei Mimura at Tohoku University in 1997. The mutant mice are backcrossed to C57BL/6. |
| Strain description | B6.129S-Ahr<tm1Yfk>. The aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediate to catalyze xenobiotic Phase I metabolism. Homozygous mutant mice shows a slightly slower growth rate compared than wild-types for the first few weeks of life. |
| Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
| References | Genes Cells, 2, 645-654 (1997). 9427285 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ahraryl-hydrocarbon receptor12Ahr<tm1Yfk>targeted mutation 1, Yoshiaki Fujii-KuriyamaAh, Ahh, Ahre, dioxin receptor, In Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)12 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)12neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)12nls |
Information2 | |
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| Donor DNA | Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 T antigen intron and polyadenylation signal, mouse Ahr genomic DNA |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Yoshiaki Fujii (University of Tsukuba) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Genetic Background Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Kimura E, Kohda M, Maekawa F, Fujii-Kuriyama Y, Tohyama C. Neurons expressing the aryl hydrocarbon receptor in the locus coeruleus and island of Calleja major are novel targets of dioxin in the mouse brain. Histochem Cell Biol (2021) 33963922 Okudaira N, Okamura T, Tamura M, Iijma K, Goto M, Matsunaga A, Ochiai M, Nakagama H, Kano S, Fujii-Kuriyama Y, Ishizaka Y. Long interspersed element-1 is differentially regulated by food-borne carcinogens via the aryl hydrocarbon receptor. Oncogene 32(41) 4903-12(2013) 23208499 |