Strain Data Sheet

RBRC01710

Strain Information

Image
BRC No.RBRC01710
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Ahr<tm1Yfk>
Former Common nameAhR(C57BL6j)-/- mouse
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Yoshiaki Fujii-Kuriyama and Junsei Mimura at Tohoku University in 1997. The mutant mice are backcrossed to C57BL/6.
Strain descriptionB6.129S-Ahr<tm1Yfk>. The aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediate to catalyze xenobiotic Phase I metabolism. Homozygous mutant mice shows a slightly slower growth rate compared than wild-types for the first few weeks of life.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
References
Loss of teratogenic response to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in mice lacking the Ah (dioxin) receptor.
Mimura J, Yamashita K, Nakamura K, Morita M, Takagi T N, Nakao K, Ema M, Sogawa K, Yasuda M, Katsuki M, Fujii-Kuriyama Y
Genes Cells, 2, 645-654 (1997). 9427285

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ahr
MGI:105043		aryl-hydrocarbon receptor12Ahr<tm1Yfk>
MGI:2657000		targeted mutation 1, Yoshiaki Fujii-Kuriyama
  • retinitis pigmentosa 85(MedGEN)
    • lacZ beta-galactosidase (E. coli)12
    neo neomycin resistance gene (E. coli)12 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    nls SV40 large T antigen nuclear localization signal (NLS)12nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal horizontal vestibuloocular reflex(MP:0011042)

  • abnormal optokinetic reflex(MP:0009619)

  • abnormal urinary bladder morphology(MP:0000538)

  • abnormal urinary bladder urothelium morphology(MP:0000540)

  • abnormal urinary bladder vasculature morphology(MP:0011773)
    • more 13 phenotypes
  • abnormal xenobiotic pharmacokinetics(MP:0008875)

  • cystolithiasis(MP:0011625)

  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • disorganized retina layers(MP:0001328)

  • homeostasis/metabolism phenotype(MP:0005376)

  • increased urinary bladder carcinoma incidence(MP:0009550)

  • increased urine uric acid level(MP:0009810)

  • nervous system phenotype(MP:0003631)

  • postnatal growth retardation(MP:0001732)

  • renal/urinary system phenotype(MP:0005367)

  • skeleton phenotype(MP:0005390)

  • urinary bladder fibrosis(MP:0011769)

  • urinary bladder inflammation(MP:0009646)
    • Detailed phenotype data

    Ordering Information

    Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 T antigen intron and polyadenylation signal, mouse Ahr genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYoshiaki Fujii (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Kimura E, Kohda M, Maekawa F, Fujii-Kuriyama Y, Tohyama C.
    Neurons expressing the aryl hydrocarbon receptor in the locus coeruleus and island of Calleja major are novel targets of dioxin in the mouse brain.
    Histochem Cell Biol 156(2) 147-163(2021) 33963922
    Okudaira N, Okamura T, Tamura M, Iijma K, Goto M, Matsunaga A, Ochiai M, Nakagama H, Kano S, Fujii-Kuriyama Y, Ishizaka Y.
    Long interspersed element-1 is differentially regulated by food-borne carcinogens via the aryl hydrocarbon receptor.
    Oncogene 32(41) 4903-12(2013) 23208499