Strain Data Sheet

RBRC01710

Information1

Image
BRC No.RBRC01710
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Ahr<tm1Yfk>
Former Common nameAhR(C57BL6j)-/- mouse
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Yoshiaki Fujii-Kuriyama and Junsei Mimura at Tohoku University in 1997. The mutant mice are backcrossed to C57BL/6.
Strain descriptionB6.129S-Ahr<tm1Yfk>. The aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediate to catalyze xenobiotic Phase I metabolism. Homozygous mutant mice shows a slightly slower growth rate compared than wild-types for the first few weeks of life.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesGenes Cells, 2, 645-654 (1997). 9427285

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ahraryl-hydrocarbon receptor12Ahr<tm1Yfk>targeted mutation 1, Yoshiaki Fujii-KuriyamaAh, Ahh, Ahre, dioxin receptor, In

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)12nls

Information2

Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, SV40 T antigen intron and polyadenylation signal, mouse Ahr genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoshiaki Fujii (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genetic Background
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Kimura E, Kohda M, Maekawa F, Fujii-Kuriyama Y, Tohyama C.
Neurons expressing the aryl hydrocarbon receptor in the locus coeruleus and island of Calleja major are novel targets of dioxin in the mouse brain.
Histochem Cell Biol (2021) 33963922

Okudaira N, Okamura T, Tamura M, Iijma K, Goto M, Matsunaga A, Ochiai M, Nakagama H, Kano S, Fujii-Kuriyama Y, Ishizaka Y.
Long interspersed element-1 is differentially regulated by food-borne carcinogens via the aryl hydrocarbon receptor.
Oncogene 32(41) 4903-12(2013) 23208499