Strain Data Sheet

RBRC01709

Strain Information

Image
BRC No.RBRC01709
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Mt1-RET)304Ina/B6
Former Common name304-B6 RET
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack with black skin (hyperpigmented skin) [a/a B/B C/C Tg/+]
black [a/a B/B C/C +/+]
Strain developmentDeveloped by Masashi Kato, Nagoya University Graduate School of Medicine. The Mt1-RET transgene was injected into the fertilized eggs of (BALB/c x C57BL/6) x BALB/c mice. C57BL/6 and BALB/c mixed background.
Strain descriptionMt1-Ret 304 transgenic mice. The mice express the human RET under the control of the mouse MT1 promoter/enhancer. This Mt1-RET 304 strain is a model of cutaneous melanosis, and develops benign tumors 100% on the skin. Of these tumors, 65％ transform to malignant tumors in ten to twelve months after the birth. Tg homozygous mice are embryonic lethal.
Colony maintenanceCarrier x Noncarrier
References
Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Iwamoto T, Takahashi M, Ito M, Hamatani K, Ohbayashi M, Wajjwalku W, Isobe K, Nakashima I
EMBO J., 10, 3167-3175 (1991). 1915289

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 splicing signal-polyadenylation siteUN
RETrecombinant RET proto-oncogene (human)UNRETmouse metallothionein 1 promoter/enhancer
Tg(Mt1-RET)304Inatransgene insertion 304, Izumi NakashimaUNTg(Mt1-RET)304Ina

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal melanogenesis(MP:0005077)

  • hyperpigmentation(MP:0001188)

  • increased cutaneous melanoma incidence(MP:0010322)

  • increased melanoma incidence(MP:0010275)

  • increased metastatic potential(MP:0001272)
    • more 2 phenotypes
  • increased tumor incidence(MP:0002020)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman RFP/RET cDNA, mouse MT-I promoter-enhancer, SV40 splicing signal-polyadenylation site, vector sequence
    Research applicationCancer Research
    Dermatology Research
    Mouse Models for Human Disease
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorMasashi Kato (Chubu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data