RBRC01695

Information1

Image
BRC No.RBRC01695
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameCNR GRv11
Former Common nameGRv11
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2004. TT2 ES cells were used.
Strain descriptionExon a11 of CNR (Pcdha) gene was replaced with a cassette containing a GAP43-HcRed, floxed PGK-neo cassette, and ires sequence. Homozygous mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesJ Biol Chem., 284, 32002-32014 (2009). 19797050

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pcdhaprotocadherin alpha cluster18Pcdha<tm7Tyag>targeted mutation 7, Takeshi Yagi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GAP43growth associated protein 4318GAP43

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HcRedRed Fluorescent Protein (Heteractis crispa)18HcRed

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, P1 phage LoxP, Heteractis crispa HcRed cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), mouse Pcdha genomic DNA, GAP43
Research applicationCell Biology Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)

BRC mice in Publications

No Data