Strain Data Sheet

RBRC01689

Strain Information

Image
BRC No.RBRC01689
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcdhg<tm(EN130)>/TyagRbrc
Former Common nameEN130, Pcdhg EN130
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, National Institute for Physiological Sciences/Graduate School of Frontier Biosciences, Osaka University in 2001. Delta neo type (RBRC03152) was generated by crossing with Sycp-cre mice.
Strain descriptionPcdhg gene knockout mice. A polyA of Pcdhg common region was replaced with tau-lacZ gene. lacZ-neo cassette type (RBRC01689), delta neo cassette type (RBRC03152). Homozygous mice show no obvious abnormality.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
References
Identification of the cluster control region for the protocadherin-beta genes located beyond the protocadherin-gamma cluster.
Yokota S, Hirayama T, Hirano K, Kaneko R, Toyoda S, Kawamura Y, Hirabayashi M, Hirabayashi T, Yagi T
J Biol Chem. , 286, 31885-31895 (2011). 21771796

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRESinternal ribosomal entry site (EMCV)18
Pcdhgprotocadherin gamma cluster18Pcdhg
lacZbeta-galactosidase (E. coli)18
loxPphage P1 loxP18loxP
loxPphage P1 loxP18loxP
neoneomycin resistance gene (E. coli)18
taubovine tau protein cDNA18tau

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse protocadherin gamma cDNA, P1 phage loxP, E. coli neo, E. coli lacZ, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bovine tau cDNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
    DepositorTakeshi Yagi (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data