Strain Data Sheet
RBRC01681
Strain Information | |
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| Image |  |
| BRC No. | RBRC01681 |
| Type | Gene Trap |
| Species | Mus musculus |
| Strain name | B6.Cg-Adhfe1<Tn(sb-lacZ,GFP)PV404Jtak>/JtakRbrc |
| Former Common name | Gene trap of BC037765 (forward orientation) & Adhfel (alcohol dehydrogenase, iron containing, 1) (reverse orientation), PV404 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system. Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J Nat. Methods, 2, 763-769 (2005). 16179923Characterization of Sleeping Beauty transposition and its application to genetic screening in mice. Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| BCL2 | Splice acceptor (human bcl-2) | 1 | BCL2 | ||||
| GFP | Green Fluorescent Protein (Aequorea victoria) | 1 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | ||||
| GH | Growth hormone polyA (human) | 1 | GH | ||||
| HBB2 | rabbit beta-globin polyA | 1 | HBB2 | ||||
| IRES | internal ribosomal entry site (EMCV) | 1 | |||||
| Pgk1 | mouse pgk promoter | 1 | Pgk1 | ||||
| SA | Splice acceptor (Salmon) | 1 | SA | ||||
| Splice donor | Splice donor (mouse hprt) | 1 | Splice donor | ||||
| Splice donor | Sleeping Beauty transposase | 1 | Splice donor | ||||
| lacZ | beta-galactosidase (E. coli) | 1 | |||||
| neo | neomycin resistance gene (E. coli) | 1 | |||||
| mouse synaptonemal protein 1 promoter | |||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | サケ由来 Sleeping Beauty トランスポゾン, サケ由来 Sleeping Beauty トランスポゼース, GFP 遺伝子, LacZ 遺伝子, neo 遺伝子,Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Human bcl-2 スプライスアクセプター, Mouse hprt スプライスドナー, Cytomegalovirus エンハンサー, Chicken beta-actin プロモーター, Mouse pgk プロモーター, Rabbit beta-globin ポリA付加シグナル, Mouse synaptonemal protein 1 プロモーター, Human growth hormone ポリ A付加シグナル, pBluescript |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
| Depositor | Junji Takeda (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |