RBRC01680

Information1

Image
BRC No.RBRC01680
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>/JtakRbrc
Former Common nameGene trap of Smoc1 (SPARC related modular calcium binding 1), PV384
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Junji Takeda at Osaka University in 2005.
Strain descriptionThis strain is a gene trap strain that have an EGFP in Smoc1 gene, produced by using the SB transposon system.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesNat. Methods, 2, 763-769 (2005). 16179923
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Smoc1SPARC related modular calcium binding 112Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>gene trap lacZ/GFP PV384, Junji Takeda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
BCL2Splice acceptor (human bcl-2)12BCL2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)12CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyA12HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)12

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pgk1mouse pgk promoter12Pgk1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptor (Salmon)12SA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Splice donorSplice donor (mouse hprt)12Splice donor

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Splice donorSleeping Beauty transposase12Splice donor

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly Ahuman growth hormone polyAdenylation signal sequence12poly A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mouse synaptonemal protein 1 promoter

Information2

Donor DNASalmon Sleeping Beauty transposon, Salmon Sleeping Beauty transposase, Jellyfish GFP gene, E. coli LacZ gene, E. coli neo gene, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human bcl-2 splice acceptor, Mouse hprt splice donor, Cytomegalovirus enhancer, Chicken beta-actin promoter, Mouse pgk promoter, Rabbit beta-globin polyadenylation signal sequence, Mouse synaptonemal protein 1 promoter, Human growth hormone polyadenylation signal sequence, pBluescript
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Takeda Lab HP

BRC mice in Publications

Okada I, Hamanoue H, Terada K, Tohma T, Megarbane A, Chouery E, Abou-Ghoch J, Jalkh N, Cogulu O, Ozkinay F, Horie K, Takeda J, Furuichi T, Ikegawa S, Nishiyama K, Miyatake S, Nishimura A, Mizuguchi T, Niikawa N, Hirahara F, Kaname T, Yoshiura K, Tsurusaki Y, Doi H, Miyake N, Furukawa T, Matsumoto N, Saitsu H.
SMOC1 is essential for ocular and limb development in humans and mice.
Am. J. Hum. Genet. 88(1) 30-41(2011) 21194678

Awwad K, Hu J, Shi L, Mangels N, Abdel Malik R, Zippel N, Fisslthaler B, Eble JA, Pfeilschifter J, Popp R, Fleming I.
Role of secreted modular calcium-binding protein 1 (SMOC1) in transforming growth factor beta signalling and angiogenesis.
Cardiovasc. Res. 106(2) 284-94(2015) 25750188