Strain Data Sheet
RBRC01680
Information1 | |
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| Image |  |
| BRC No. | RBRC01680 |
| Type | Gene Trap |
| Species | Mus musculus |
| Strain name | B6.Cg-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>/JtakRbrc |
| Former Common name | Gene trap of Smoc1 (SPARC related modular calcium binding 1), PV384 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Junji Takeda at Osaka University in 2005. |
| Strain description | This strain is a gene trap strain that have an EGFP in Smoc1 gene, produced by using the SB transposon system. |
| Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
| References | Nat. Methods, 2, 763-769 (2005). 16179923 Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Smoc1SPARC related modular calcium binding 112Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>gene trap lacZ/GFP PV384, Junji Takeda Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter BCL2Splice acceptor (human bcl-2)12BCL2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)12CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (human)12GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA12HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)12internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pgk1mouse pgk promoter12Pgk1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (Salmon)12SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Splice donorSleeping Beauty transposase12Splice donor Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Splice donorSplice donor (mouse hprt)12Splice donor Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)12 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)12neo; neomycin; Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mouse synaptonemal protein 1 promoter |
Information2 | |
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| Donor DNA | Salmon Sleeping Beauty transposon, Salmon Sleeping Beauty transposase, Jellyfish GFP gene, E. coli LacZ gene, E. coli neo gene, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Human bcl-2 splice acceptor, Mouse hprt splice donor, Cytomegalovirus enhancer, Chicken beta-actin promoter, Mouse pgk promoter, Rabbit beta-globin polyadenylation signal sequence, Mouse synaptonemal protein 1 promoter, Human growth hormone polyadenylation signal sequence, pBluescript |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
| Depositor | Junji Takeda (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Okada I, Hamanoue H, Terada K, Tohma T, Megarbane A, Chouery E, Abou-Ghoch J, Jalkh N, Cogulu O, Ozkinay F, Horie K, Takeda J, Furuichi T, Ikegawa S, Nishiyama K, Miyatake S, Nishimura A, Mizuguchi T, Niikawa N, Hirahara F, Kaname T, Yoshiura K, Tsurusaki Y, Doi H, Miyake N, Furukawa T, Matsumoto N, Saitsu H. SMOC1 is essential for ocular and limb development in humans and mice. Am J Hum Genet 88(1) 30-41(2011) 21194678 Delgado Lagos FA, Elgheznawy A, Kyselova A, Meyer Zu Heringdorf D, Ratiu C, Randriamboavonjy V, Mann AW, Fisslthaler B, Siragusa M, Fleming I. Secreted modular calcium binding protein 1 binds/activates thrombin to account for platelet hyper-reactivity in diabetes. Blood (2021) 33529332 Awwad K, Hu J, Shi L, Mangels N, Abdel Malik R, Zippel N, Fisslthaler B, Eble JA, Pfeilschifter J, Popp R, Fleming I. Role of secreted modular calcium-binding protein 1 (SMOC1) in transforming growth factor β signalling and angiogenesis. Cardiovasc Res 106(2) 284-94(2015) 25750188 |