Strain Data Sheet

RBRC01662

Strain Information

Image
BRC No.RBRC01662
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Grm1<tm1.1(GFP)>/Rrbc
Former Common namemGluR1 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Drs. Tatsumi Hirata and Yumiko Saga in National Institute of Genetics at 2005. PGK-neo flanked by loxP sites linked to a EGFP gene was inserted into the second exon of Grm1 gene of TT2 ES cells. The expression of the EGFP was unknown. Pgk-neo casette was removed.
Strain descriptionGrm1 (glutamate receptor, metabotropic 1) is a member of a large family of G-protein-coupled glutamate receptor that is important for normal synapse formation, synaptic plasticity, and motor control in the cerebellum. Mice deficient in mGluR1 have severe phenotype in motor coordination.
Colony maintenanceSibling Mating (Heterozygote x Wild-type, Heterozygote)Homozygous mutant mice show severe phenotype in motor coordination in 10-14 days old.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)10
  • autosomal recessive spinocerebellar ataxia 13(DisGeNET, MedGEN)

  • spinocerebellar ataxia 44(MedGEN)
    • Grm1glutamate receptor, metabotropic 110Grm1
  • autosomal recessive spinocerebellar ataxia 13(DisGeNET, MedGEN)

  • spinocerebellar ataxia 44(MedGEN)
    • loxPphage P1 loxP10loxP
  • autosomal recessive spinocerebellar ataxia 13(DisGeNET, MedGEN)

  • spinocerebellar ataxia 44(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cerebellar plate morphology(MP:0000856)

  • ataxia(MP:0001393)

  • reduced fertility(MP:0001921)
    • Detailed phenotype data

    Ordering Information

    Donor DNAjellyfish enhanced green fluorescent protein gene, phage P1 loxP, mouse mGluR1 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorTastumi Hirata (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Fujinaga M, Yamasaki T, Yui J, Hatori A, Xie L, Kawamura K, Asagawa C, Kumata K, Yoshida Y, Ogawa M, Nengaki N, Fukumura T, Zhang MR.
    Synthesis and evaluation of novel radioligands for positron emission tomography imaging of metabotropic glutamate receptor subtype 1 (mGluR1) in rodent brain.
    J Med Chem 55(5) 2342-52(2012) 22316010