Strain Data Sheet

RBRC01659

Strain Information

Image
BRC No.RBRC01659
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc
Former Common nameBALB/c-CD11c-DTR
H-2 Haplotype
ES Cell line
Background strainBALB/cAnNCrlCrlj
Appearance
Strain developmentDeveloped by Steffen Jung in 2002. DTR/GFP under the control of the mouse Itgax promoer. The DTR/GFP1 fusion gene under the mouse Itgax promoter was injected into the pronuclei of FVB/N fertilized eggs.
Strain descriptionThis transgenic mice has a diphtheria toxin recepotr (DTR) and GFP fusion gene under under the control of the mouse Itgax promoer. The dendritic cells of this strain were transiently disrupted with DT inducible system.
Colony maintenance
References
In vivo depletion of CD11c+ dendritic cells abrogates priming of CD8+ T cells by exogenous cell-associated antigens.
Jung S, Unutmaz D, Wong P, Sano G, De los Santos K, Sparwasser T, Wu S, Vuthoori S, Ko K, Zavala F, Pamer E G, Littman D R, Lang R A
Immunity, 17, 211-220 (2002). 12196292

Health Report

Examination Date / Room / Rack2024/05/27Room:4-BRack:I
2024/02/26Room:4-BRack:I

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UN
HBEGFheparin-binding EGF-like growth factor (human)UNHBEGFmouse Itgax promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Itgax promoter, monkey heparin-binding EGF-like growth factor(hbEGF), jellyfish Green Fluorescence Protein (GFP) cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Dan Littman).
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data