Strain Data Sheet

RBRC01653

Strain Information

Image
BRC No.RBRC01653
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-sb10)1Jtak/JtakRbrc
Former Common nameCAG-SB10, B6.Cg-Tg(ACTB-sb10)1Jtak
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack
Strain developmentDeveloped by Drs. Kyoji Horie and Junji Takeda at Osaka University in 2001. The CX-SB plasmid was injected into the pronuclei of B6C3F2 fertilized egges. C57BL/6, C3H and ICR mixed background.
Strain descriptionThis transgenic mice express Sleeping Beauty (SB) transposase under control of CAG promotor. The double transgenic mice containing SB transposase and SB transposon vector, generated by crossing with this Tg mice and the Tg mice having SB transposon vector, can be used to generate many mutant mice through germline transmission by breeding with wild-type mouse.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6J)
References
Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Efficient chromosomal transposition of a Tc1/mariner- like transposon Sleeping Beauty in mice.
Horie K, Kuroiwa A, Ikawa M, Okabe M, Kondoh G, Matsuda Y, Takeda J
Proc. Natl. Acad. Sci. USA, 98, 9191-9196 (2001). 11481482

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SBSleeping Beauty transposaseUNSBCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), salmonid Sleeping Beauty Transposase
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data