RBRC01653

Information1

Image
BRC No.RBRC01653
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameCX-SB
Former Common nameB6.Cg-Tg(ACTB-sb10)1Jtak
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack
Strain developmentDeveloped by Drs. Kyoji Horie and Junji Takeda at Osaka University in 2001. The CX-SB plasmid was injected into the pronuclei of B6C3F2 fertilized egges. C57BL/6, C3H and ICR mixed background.
Strain descriptionThis transgenic mice express Sleeping Beauty (SB) transposase under control of CAG promotor. The double transgenic mice containing SB transposase and SB transposon vector, generated by crossing with this Tg mice and the Tg mice having SB transposon vector, can be used to generate many mutant mice through germline transmission by breeding with wild-type mouse.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6J)
ReferencesNat. Methods, 2, 763-769 (2005). 16179923
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530
Proc. Natl. Acad. Sci. USA, 98, 9191-9196 (2001). 11481482

Health Report

Examination Date / Room / Rack2008/03/17Room:4-ARack:D
2008/01/14Room:4-ARack:D
2007/11/12Room:4-ARack:D
2007/09/18Room:4-ARack:D
2007/07/17Room:4-ARack:D
2007/05/22Room:4-ARack:D
2007/05/21Room:4-ARack:D
2007/03/19Room:4-ARack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
SBSleeping Beauty transposaseUNSBCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Sleeping Beauty Transposase
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data