RBRC01652

Information1

Image
BRC No.RBRC01652
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameIF2
Former Common nameSB transposon IF2
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack
Strain developmentDeveloped by Drs. Kyoji Horie and Junji Takeda at Osaka University in 2004. The SB transposon vecotor consists of a GFP expression cassette flanked by a splice donor and a lacZ cassette flanked by acceptor, allowing for poly (A)-trap selection of transposition events and promoter-trap for endogenous gene expression pattern profiling , respectively. This transposon vector was injected into the pronuclei of B6D2F2 fertilized egges. C57BL/6, DBA/2 and ICR mixed background.
Strain descriptionThis transgenic mouse strain has a Sleeping Beauty (SB) transposon vector acting as a mutagen. The double transgenic mice containing SB transposon vector and SB transposase, generated by crossing with this Tg mice and the Tg mice having SB transposase, can be used to generate many mutant mice through germline transmission by breeding with wild-type mouse.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6J)
ReferencesMol. Cell. Biol., 23, 9189-9207 (2003). 14645530
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack2007/07/17Room:4-ARack:G
2007/05/22Room:4-ARack:G
2007/05/21Room:4-ARack:G
2007/03/19Room:4-ARack:G
2007/01/15Room:4-ARack:G
2006/11/13Room:4-ARack:G
2006/09/19Room:4-ARack:G
2006/09/19Room:4-ARack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SASplice acceptorUNSA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Splice donorSplice donorUNSplice donor

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)UNmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Information2

Donor DNApTrans-SA-IRESLacZ-CAG-GFP-SD:Neo[SA (The human bcl-2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, Simian virus 40 Large T antigen nuclear localization signal (nls), CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), EGFP, SD(splice donor sequences from the mouse hprt gene), E.coli NEO, the mRNA instability signal derived from the 3′ untranslated region of the human granulocyte-macrophage colony-stimulating factor cDNA], mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Takeda Lab HP

BRC mice in Publications

Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.
Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
Exp. Anim. 58(4) 437-42(2009) 19654444