RBRC01624

Information1

Image
BRC No.RBRC01624
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc
Former Common name
H-2 Haplotype
ES Cell line
Background strainBALB/cAJcl
Appearance
Strain development
Strain descriptionThese transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. Both homozygous and hemizygous transgenic mice express GFP in all over the body. This strain is useful for detection of cell fate in transplantation and developmental studies. Homozygous transgenic females experienced show a high incidence of sudden death (about 30%), putatively due to dilated cardiomyopaty. Transgene integration site: 14D1.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2015/07/27Room:4-BRack:H
2015/05/25Room:4-BRack:H
2015/03/23Room:4-BRack:H
2015/01/19Room:4-BRack:H
2014/11/17Room:4-BRack:H
2014/09/16Room:4-BRack:H
2014/07/14Room:4-BRack:H
2014/05/19Room:4-BRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)14CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the Department of Experimental Genome Research. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP
Mouse of the Month Feb 2012
Genetic Background

BRC mice in Publications

No Data