Strain Data Sheet

RBRC01624

Strain Information

Image
BRC No.RBRC01624
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc
Former Common name
H-2 Haplotype
ES Cell line
Background strainBALB/cAJcl
Appearance
Strain developmentThis strain was generated in C57BL/6 mice by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University (RBRC00267 C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb). The mice were then backcrossed to BALB/cANMs (RBRC01624 C.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc), C3H/HeNCrlCrlj (RBRC01624 C3.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc), MSM/MsRbrc (RBRC01626 MSM;B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Rbrc) and DBA/2CrSlc (RBRC02889 D2.B6-Tg(CAG-EGFP)C14-Y01-FM131Osb/Ny), respectively. Congenic strains of BALB/c, C3H and MSM background were developed by RIKEN BioResource Center, and DBA/2 congenic strain was developed by Naoki Yamamoto, Fijita Health University.
Strain descriptionThis transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. Both homozygous and hemizygous transgenic mice express GFP in all over the body. This strain is useful for detection of cell fate in transplantation and developmental studies. Tg hemizygous and homozygous mice are viable and fertile.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/05/27Room:4-BRack:A
2024/02/26Room:4-BRack:A
2023/11/27Room:4-BRack:A

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)14CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the Department of Experimental Genome Research. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Feb 2012

    BRC mice in Publications

    No Data