RBRC01601

Information1

Image
BRC No.RBRC01601
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S7-Tert<tm1Fish>/FishRbrc
Former Common namemTERT KO mice
H-2 Haplotype
ES Cell lineJH1 [129S7/SvEvBrd]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Xunmei Yuan and Fuyuki Ishikawa at Tokyo Institute of Technology in 1999. A neomycin selection cassette replaced three exons, encoding a highly conserved motif A of the Tert gene. The mutant mice were backcrossed C57BL/6N.
Strain descriptionTert, telomerase reverse transcriptase gene encodes a catalytic subunit of telomerase that is essential for telomerase activity. Homozygous mutant mice show no telomerase activity. Homozygous mutant mice after 3 to 5 generations were sterile.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj) Homozygous mutant mice after 3 to 5 generations were sterile.
ReferencesGenes Cells, 4, 563-572 (1999). 10583505

Health Report

Examination Date / Room / Rack2011/01/17Room:4-ARack:H
2010/11/16Room:4-ARack:H
2010/09/13Room:4-ARack:H
2010/07/12Room:4-ARack:H
2010/05/17Room:4-ARack:H
2010/03/15Room:4-CRack:F
2006/09/19Room:4-ARack:H
2006/09/19Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Terttelomerase reverse transcriptase13Tert<tm1Fish>targeted mutation 1, Fuyuki Ishikawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)13GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13mouse RNA polymenase II promoter, bovine growth hormone poly A signal

Information2

Donor DNAmouse RNA polymenase II promoter, E. coli neo, bovine growth hormone polyA signal, mouse TERT genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes to Cells, 4, 563-572 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Recipient agrees to use the Biological Resource for academic use, shall not use for commercial use.
DepositorFuyuki Ishikawa (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data