Strain Data Sheet

RBRC01601

update : 2024-08-20

Strain Information
Image
BRC No.	RBRC01601
Type	Targeted Mutation Congenic
Species	Mus musculus
Strain name	B6.129S7-Tert<tm1Fish>/FishRbrc
Former Common name	mTERT KO mice
H-2 Haplotype
ES Cell line	JH1 [129S7/SvEvBrd]
Background strain	C57BL/6NCrlCrlj
Appearance	black [a/a B/B C/C]
Strain development	Developed by Drs. Xunmei Yuan and Fuyuki Ishikawa at Tokyo Institute of Technology in 1999. A neomycin selection cassette replaced three exons, encoding a highly conserved motif A of the Tert gene. The mutant mice were backcrossed C57BL/6N.
Strain description	Tert, telomerase reverse transcriptase gene encodes a catalytic subunit of telomerase that is essential for telomerase activity. Homozygous mutant mice show no telomerase activity. Homozygous mutant mice after 3 to 5 generations were sterile.
Colony maintenance	Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj) Homozygous mutant mice after 3 to 5 generations were sterile.
References	Presence of telomeric G-strand tails in the telomerase catalytic subunit TERT knockout mice. Yuan X, Ishibashi S, Hatakeyama S, Saito M, Nakayama J, Nikaido R, Haruyama T, Watanabe Y, Iwata H, Iida M, Sugimura H, Yamada N, Ishikawa F Genes Cells, 4, 563-572 (1999). 10583505

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
GH	Growth hormone polyA (Bovine)	13	GH				acute myeloid leukemia(MedGEN) aplastic anemia(MedGEN) dyskeratosis congenita, autosomal dominant 1(MedGEN) more 3 Diseases dyskeratosis congenita, autosomal dominant 2(MedGEN) melanoma, cutaneous malignant, susceptibility to, 9(MedGEN) pulmonary fibrosis and/or bone marrow failure, Telomere-related, 1(MedGEN)
Tert	telomerase reverse transcriptase	13	Tert	targeted mutation 1, Fuyuki Ishikawa			acute myeloid leukemia(MedGEN) aplastic anemia(MedGEN) dyskeratosis congenita, autosomal dominant 1(MedGEN) more 3 Diseases dyskeratosis congenita, autosomal dominant 2(MedGEN) melanoma, cutaneous malignant, susceptibility to, 9(MedGEN) pulmonary fibrosis and/or bone marrow failure, Telomere-related, 1(MedGEN)
neo	neomycin resistance gene (E. coli)	13				mouse RNA polymenase II promoter, bovine growth hormone poly A signal	acute myeloid leukemia(MedGEN) aplastic anemia(MedGEN) dyskeratosis congenita, autosomal dominant 1(MedGEN) more 3 Diseases dyskeratosis congenita, autosomal dominant 2(MedGEN) melanoma, cutaneous malignant, susceptibility to, 9(MedGEN) pulmonary fibrosis and/or bone marrow failure, Telomere-related, 1(MedGEN)

Phenotype
Annotation by Mammalian phenotyhpe ontology	reduced fertility(MP:0001921)
Detailed phenotype data

Ordering Information
Donor DNA	mouse RNA polymenase II promoter, E. coli neo, bovine growth hormone polyA signal, mouse TERT genomic DNA
Research application
Specific Term and Conditions	The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes to Cells, 4, 563-572 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The Recipient agrees to use the Biological Resource for academic use, shall not use for commercial use.
Depositor	Fuyuki Ishikawa (Kyoto University)
Strain Status	Frozen embryos Frozen sperm
Strain Availability	Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month)
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP (Japanese) Genotyping protocol -PCR-

BRC mice in Publications

No Data