Strain Data Sheet

RBRC01597

Strain Information

Image
BRC No.RBRC01597
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(KRT14-Ahr-CA)234Mym/MymRbrc
Former Common nameK14-AhR-CA TG mouse line 234, B6D2-Tg(hK14-AhR-CA)234Mym/MymRbrc
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearancedilute black [a/a B/? C/C d/d]
black [a/a B/? C/C D/?]
brown [a/a b/b C/C D/D]
dilute brown [a/a b/b C/C d/d]
Strain developmentDeveloped by Dr. Masayuki Yamamoto at Tsukuba University in 2002. A cDNA fragment encording the constitutive active form of mouse AhR (AhR-CA) under the human cytokeratin 14 promoter was injected into the pronuclei of fertilized egges of B6D2F1. The 234 line mice were maintained by hemizygous female x B6D2F1 wild-type male mating.
Strain descriptionK14-AhR-CA transgenic mice carry a cDNA fragment encording the constitutive active form of mouse AhR (aryl hydrocarbon receptor) under control of human cytokeratin 14 promoter. The aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediate to catalyze xenobiotic Phase I metabolism. The transgene was integrated into the X chromosome in the Line 234. Tg hemizygous mice are normal at birth, but severe skin lesions with itching develop postnatally. Tg hemizygous males die before weaning. This strain is useful for the evaluation of the causal relationship between the AhR gene expression and inflammatory dermatitis.
Colony maintenanceCross to B6D2F1 (Hemizygous female x Jcl:B6D2F1 male)Tg hemizygote mice are normal at birth, but severe skin lesions with itching develop postnatally, and the Tg males die before weaning.
References
Constitutive expression of aryl hydrocarbon receptor in keratinocytes causes inflammatory skin lesions.
Tauchi M, Hida A, Negishi T, Katsuoka F, Noda S, Mimura J, Hosoya T, Yanaka A, Aburatani H, Fujii-Kuriyama Y, Motohashi H, Yamamoto M
Mol. Cell. Biol., 25, 9360-9368 (2005). 16227587

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ahraryl-hydrocarbon receptorUNAhrhuman Keratin14 promoter
  • dermatopathia pigmentosa reticularis(MedGEN)

  • epidermolysis bullosa simplex 1D, generalized, intermediate or severe, autosomal recessive(MedGEN)

  • epidermolysis bullosa simplex 1A, generalized severe(MedGEN)
    • more 3 Diseases
  • epidermolysis bullosa simplex 1C, localized(MedGEN)

  • Naegeli-Franceschetti-Jadassohn syndrome(MedGEN)

  • retinitis pigmentosa 85(MedGEN)
    • HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA
  • dermatopathia pigmentosa reticularis(MedGEN)

  • epidermolysis bullosa simplex 1D, generalized, intermediate or severe, autosomal recessive(MedGEN)

  • epidermolysis bullosa simplex 1A, generalized severe(MedGEN)
    • more 3 Diseases
  • epidermolysis bullosa simplex 1C, localized(MedGEN)

  • Naegeli-Franceschetti-Jadassohn syndrome(MedGEN)

  • retinitis pigmentosa 85(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal adipose tissue physiology(MP:0005666)

  • abnormal circulating chemokine level(MP:0010209)

  • abnormal grooming behavior(MP:0001440)

  • abnormal keratinocyte differentiation(MP:0002656)

  • abnormal keratinocyte morphology(MP:0002655)
    • more 15 phenotypes
  • abnormal pruritus(MP:0010071)

  • abnormal skin condition(MP:0001191)

  • acanthosis(MP:0001874)

  • adipose tissue inflammation(MP:0020386)

  • dermatitis(MP:0001194)

  • excessive scratching(MP:0001412)

  • increased IgE level(MP:0002497)

  • increased IgG level(MP:0002493)

  • increased circulating interferon-beta level(MP:0008575)

  • increased inflammatory response(MP:0001846)

  • increased interleukin-18 secretion(MP:0008684)

  • increased mast cell number(MP:0000324)

  • increased pruritus(MP:0010072)

  • skin lesions(MP:0001212)

  • spontaneous skin ulceration(MP:0001209)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman keratin14 promoter, mouse AhR cDNA, HA (hemagglutinin) tag sequence, human KRT14 polyA
    Research applicationDermatology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 25, 9360-9368 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasayuki Yamamoto (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-
    Mouse of the Month Aug 2007

    BRC mice in Publications

    No Data