RBRC01597

Information1

Image
BRC No.RBRC01597
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(hK14-AhR-CA)234Mym/MymRbrc
Former Common nameK14-AhR-CA TG mouse line 234
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearancedilute brown [a/a b/b C/C d/d]
dilute black [a/a B/? C/C d/d]
black [a/a B/? C/C D/?]
brown [a/a b/b C/C D/D]
Strain developmentDeveloped by Dr. Masayuki Yamamoto at Tsukuba University in 2002. A cDNA fragment encording the constitutive active form of mouse AhR (AhR-CA) under the human cytokeratin 14 promoter was injected into the pronuclei of fertilized egges of B6D2F1. The 234 line mice were maintained by hemizygous female x B6D2F1 wild-type male mating.
Strain descriptionK14-AhR-CA transgenic mice carry a cDNA fragment encording the constitutive active form of mouse AhR (aryl hydrocarbon receptor) under control of human cytokeratin 14 promoter. The aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediate to catalyze xenobiotic Phase I metabolism. The transgene was integrated into the X chromosome in the Line 234. Tg hemizygous mice are normal at birth, but severe skin lesions with itching develop postnatally. Tg hemizygous males die before weaning. This strain is useful for the evaluation of the causal relationship between the AhR gene expression and inflammatory dermatitis.
Colony maintenanceCross to B6D2F1 (Hemizygous female x Jcl:B6D2F1 male)Tg hemizygote mice are normal at birth, but severe skin lesions with itching develop postnatally, and the Tg males die before weaning.
ReferencesMol. Cell. Biol., 25, 9360-9368 (2005). 16227587

Health Report

Examination Date / Room / Rack2011/01/11Room:2-検Rack:M
2010/11/08Room:2-検Rack:M
2010/09/06Room:2-検Rack:M
2008/03/03Room:2-検Rack:K
2007/10/29Room:2-検Rack:K
2007/07/02Room:2-検Rack:K
2007/05/08Room:2-検Rack:K
2007/05/07Room:2-検Rack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ahraryl-hydrocarbon receptorUNAhrhuman Keratin14 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ApolyAUNpoly A

Information2

Donor DNAhuman keratin14, mouse AhR cDNA,HA (hemagglutinin) tag sequence, poly A
Research applicationDermatology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 25, 9360-9368 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayuki Yamamoto (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Aug 2007

BRC mice in Publications

No Data