RBRC01593

Information1

Image
BRC No.RBRC01593
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Bcl2<tm1Tsu>/TsuRbrc
Former Common namebcl-2 knockout mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Drs. Kamada Shinji and Yoshihide Tsujimoto at Osaka University Medical School in 1994. A neomycin selection cassette replaced most of coding sequences of exons 2 of the Bcl2 gene. The mutant mice were backcrossed C57BL/6J.
Strain descriptionB6.129P2-Bcl2<tm1Tsu>. Bcl2, B-cell leukemia/lymphoma 2 gene is a major regulator of programmed cell death, initially identified through study of the t(14;18) chromosomal translocation in human B cell follicular lymphomas. This gene is expressed in variety of tissues and cell types in embryos and adults. Most of homozygous mutant mice displays growth retardation, smaller ears, short lives, polycystic kidney, atrophic thymus and sleep with accelerated apoptotic cell death of lymphocyes, and hair hypopigmentaion in the second hair follicle cycle.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesCancer Res., 55, 354-359 (1995). 7812968

Health Report

Examination Date / Room / Rack2019/07/08Room:2-検Rack:F
2019/05/07Room:2-検Rack:F
2019/03/11Room:2-検Rack:F
2019/01/15Room:2-検Rack:F
2018/11/12Room:2-検Rack:F
2018/09/10Room:2-検Rack:F
2018/07/09Room:2-検Rack:F
2018/05/14Room:2-検Rack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Bcl2B-cell leukemia/lymphoma 21Bcl2<tm1Tsu>targeted mutation 1, Yoshihide Tsujimoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyA, mouse Bcl-2 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Kamada S, Shimono A, Shinto Y, Tsujimura T, Takahashi T, Noda T, Kitamura Y, Kondoh H, Tsujimoto Y. bcl-2 deficiency in mice leads to pleiotropic abnormalities: accelerated lymphoid cell death in thymus and spleen, polycystic kidney, hair hypopigmentation, and distorted small intestine. Cancer Res. 1995 Jan 15;55(2):354-9.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the indicated literature and an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorYoshihide Tsujimoto (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data