Strain Data Sheet
RBRC01593
Strain Information | |
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| Image |  |
| BRC No. | RBRC01593 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Bcl2<tm1Tsu>/TsuRbrc |
| Former Common name | bcl-2 knockout mouse |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Drs. Kamada Shinji and Yoshihide Tsujimoto at Osaka University Medical School in 1994. A neomycin selection cassette replaced most of coding sequences of exons 2 of the Bcl2 gene. The mutant mice were backcrossed C57BL/6J. |
| Strain description | B6.129P2-Bcl2<tm1Tsu>. Bcl2, B-cell leukemia/lymphoma 2 gene is a major regulator of programmed cell death, initially identified through study of the t(14;18) chromosomal translocation in human B cell follicular lymphomas. This gene is expressed in variety of tissues and cell types in embryos and adults. Most of homozygous mutant mice displays growth retardation, smaller ears, short lives, polycystic kidney, atrophic thymus and sleep with accelerated apoptotic cell death of lymphocyes, and hair hypopigmentaion in the second hair follicle cycle. |
| Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
| References | bcl-2 deficiency in mice leads to pleiotropic abnormalities: accelerated lymphoid cell death in thymus and spleen, polycystic kidney, hair hypopigmentation, and distorted small intestine. Kamada S, Shimono A, Shinto Y, Tsujimura T, Takahashi T, Noda T, Kitamura Y, Kondoh H, Tsujimoto Y Cancer Res., 55, 354-359 (1995). 7812968 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Bcl2 | B cell leukemia/lymphoma 2 | 1 | Bcl2 | targeted mutation 1, Yoshihide Tsujimoto | |||
| neo | neomycin resistance gene (E. coli) | 1 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 18 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyA, mouse Bcl-2 genomic DNA |
| Research application | Immunology and Inflammation Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Kamada S, Shimono A, Shinto Y, Tsujimura T, Takahashi T, Noda T, Kitamura Y, Kondoh H, Tsujimoto Y. bcl-2 deficiency in mice leads to pleiotropic abnormalities: accelerated lymphoid cell death in thymus and spleen, polycystic kidney, hair hypopigmentation, and distorted small intestine. Cancer Res. 1995 Jan 15;55(2):354-9.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the indicated literature and an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. |
| Depositor | Yoshihide Tsujimoto (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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