Strain Data Sheet



BRC No.RBRC01578
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pik3r1<tm1Tka>/Rbrc
Former Common nameC57BL/6-PI3K KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Kadowaki of the University of Tokyo and backcrossed to C57BL/6 for 12 generations by depositor.
Strain descriptionPI3K, phosphoinositide-3-kinase gene is a lipid kinases that catalyze the addition of a phosphate molecule specifically to the 3-position of the inositol ring of phosphoinositides. This strain is useful for elucidating of the rule of PI3K in various phases of immune responses. Homozygous mutant females were sterile.
Colony maintenanceSince most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males.
ReferencesScience, 283, 390-392 (1999). 9888854
Nat. Genet., 21, 230-235 (1999). 9988280

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pik3r1phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)13Pik3r1<tm1Tka>targeted mutation 1, Takashi Kadowakip50alpha, p55alpha, p85alpha, PI3K

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13neo; neomycin;herpes simplex virus thymidine kinase (HSV TK) poly A


Donor DNAE. coli neo, HSV TK polyA, mouse PI3K genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data