RBRC01577

Information1

Image
BRC No.RBRC01577
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Pik3r1<tm1Tka>/Rbrc
Former Common nameBALB/c-PI3K KO
H-2 Haplotype
ES Cell line
Background strainBALB/cAnNCrlCrlj
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Kadowaki of the University of Tokyo and backcrossed to BALB/c for 12 generations by depositor.
Strain descriptionC.Cg-Pik3r1<tm1Tka>. The PI3K, phosphoinositide-3-kinase gene is a key enzyme producing phospholipid second messengers and plays an important role in various signal transduction pathways. This knockout mouse strain is useful for investigating the function of the PI3K gene.
Colony maintenanceSince most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males.
ReferencesScience, 283, 390-392 (1999). 9888854
Nat. Genet., 21, 230-235 (1999). 9988280

Health Report

Examination Date / Room / Rack2010/01/18Room:4-BRack:L
2009/11/16Room:4-BRack:L
2009/09/14Room:4-BRack:L
2009/07/13Room:4-BRack:L
2009/05/19Room:4-BRack:L
2009/05/18Room:4-BRack:L
2009/03/16Room:4-BRack:L
2009/01/19Room:4-BRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pik3r1phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)13Pik3r1<tm1Tka>targeted mutation 1, Takashi Kadowaki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly AHSV TK polyA13poly A

Information2

Donor DNAmouse PI3K genomic DNA, E. coli neo, HSV TK polyA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Umehara D, Watanabe S, Ochi H, Anai Y, Ahmed N, Kannagi M, Hanson C, Ruscetti S, Nishigaki K.
Role of phosphatidylinositol 3-kinase in friend spleen focus-forming virus-induced erythroid disease.
J. Virol. 84(15) 7675-82(2010) 20504929