Strain Data Sheet

RBRC01577

Strain Information

Image
BRC No.RBRC01577
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Pik3r1<tm1Tka>/Rbrc
Former Common nameBALB/c-PI3K KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cAnNCrlCrlj
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Kadowaki of the University of Tokyo and backcrossed to BALB/c for 12 generations by depositor.
Strain descriptionC.Cg-Pik3r1<tm1Tka>. The PI3K, phosphoinositide-3-kinase gene is a key enzyme producing phospholipid second messengers and plays an important role in various signal transduction pathways. This knockout mouse strain is useful for investigating the function of the PI3K gene.
Colony maintenanceSince most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males.
References
Xid-like immunodeficiency in mice with disruption of the p85alpha subunit of phosphoinositide 3-kinase.
Suzuki H, Terauchi Y, Fujiwara M, Aizawa S, Yazaki Y, Kadowaki T, Koyasu S
Science, 283, 390-392 (1999). 9888854

Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase.
Terauchi Y, Tsuji Y, Satoh S, Minoura H, Murakami K, Okuno A, Inukai K, Asano T, Kaburagi Y, Ueki K, Nakajima H, Hanafusa T, Matsuzawa Y, Sekihara H, Yin Y, Barrett J C, Oda H, Ishikawa T, Akanuma Y, Komuro I, Suzuki M, Yamamura K, Kodama T, Suzuki H, Yamamura K, Kodama T, Suzuki H, Koyasu S, Aizawa S, Tobe K, Fukui Y, Yazaki Y, Kadowaki T
Nat. Genet., 21, 230-235 (1999). 9988280

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pik3r1phosphoinositide-3-kinase regulatory subunit 113Pik3r1targeted mutation 1, Takashi Kadowaki
  • agammaglobulinemia 7, autosomal recessive(MedGEN)

  • immunodeficiency 36(MedGEN)

  • SHORT syndrome(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)13
  • agammaglobulinemia 7, autosomal recessive(MedGEN)

  • immunodeficiency 36(MedGEN)

  • SHORT syndrome(DisGeNET, MedGEN)
    • poly AHSV TK polyA13poly A
  • agammaglobulinemia 7, autosomal recessive(MedGEN)

  • immunodeficiency 36(MedGEN)

  • SHORT syndrome(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell physiology(MP:0002459)

  • abnormal adipocyte glucose uptake(MP:0004185)

  • abnormal humoral immune response(MP:0001800)

  • abnormal muscle cell glucose uptake(MP:0004130)

  • decreased B cell number(MP:0005017)
    • more 12 phenotypes
  • decreased B cell proliferation(MP:0005093)

  • decreased circulating glucose level(MP:0005560)

  • decreased circulating insulin level(MP:0002727)

  • decreased mature B cell number(MP:0008211)

  • decreased pre-B cell number(MP:0008209)

  • decreased pro-B cell number(MP:0008208)

  • hypoglycemia(MP:0000189)

  • increased adipocyte glucose uptake(MP:0030016)

  • increased insulin sensitivity(MP:0002891)

  • increased muscle cell glucose uptake(MP:0030021)

  • increased susceptibility to bacterial infection(MP:0002412)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse PI3K genomic DNA, E. coli neo, HSV TK polyA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 283, 390-392 (1999). Nat. Genet., 21, 230-235 (1999).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    Umehara D, Watanabe S, Ochi H, Anai Y, Ahmed N, Kannagi M, Hanson C, Ruscetti S, Nishigaki K.
    Role of phosphatidylinositol 3-kinase in friend spleen focus-forming virus-induced erythroid disease.
    J Virol 84(15) 7675-82(2010) 20504929