Strain Data Sheet

RBRC01560

Strain Information

Image
BRC No.RBRC01560
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Slc25a21<Tn(sb-lacZ,GFP)PV200Jtak>/JtakRbrc
Former Common namePV200
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)12CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
  • mitochondrial DNA depletion syndrome 18(MedGEN)
    • IRESinternal ribosomal entry site (EMCV)12
  • mitochondrial DNA depletion syndrome 18(MedGEN)
    • SASplice acceptor12SA
  • mitochondrial DNA depletion syndrome 18(MedGEN)
    • Slc25a21solute carrier family 25 (mitochondrial oxodicarboxylate carrier), member 2112Slc25a21transposon insertion PV200, Junji Takeda
  • mitochondrial DNA depletion syndrome 18(MedGEN)
    • Splice donorSplice donor12Splice donor
  • mitochondrial DNA depletion syndrome 18(MedGEN)
    • lacZbeta-galactosidase (E. coli)12
  • mitochondrial DNA depletion syndrome 18(MedGEN)
    • neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter)
  • mitochondrial DNA depletion syndrome 18(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • embryonic lethality(MP:0008762)
    • Detailed phenotype data

    Ordering Information

    Donor DNApTrans-SA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), lacZ-CAG-GFP-SD: NEO, EGFP, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data