Strain Data Sheet

RBRC01556

Strain Information

Image
BRC No.RBRC01556
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nell2<Tn(sb-lacZ,GFP)OR238Jtak>/JtakRbrc
Former Common nameGene trap of Nell2 (nel-like 2 homolog), OR238
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
BCL2Splice acceptor (human bcl-2)15BCL2
GFPGreen Fluorescent Protein (Aequorea victoria)15CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
GHGrowth hormone polyA (human)15GH
HBB2rabbit beta-globin polyA15HBB2
IRESinternal ribosomal entry site (EMCV)15
Nell2NEL-like 215Nell2transposon insertion OR238, Junji Takeda
SBSleeping Beauty transposon15SB
Splice donorSplice donor (mouse hprt)15Splice donor
lacZbeta-galactosidase (E. coli)15
neoneomycin resistance gene (E. coli)15

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNASalmon Sleeping Beauty transposon*, Salmon Sleeping Beauty transposase*, Human bcl-2 splice acceptor, Encephalomyocarditis virus (EMCV)internal ribosomal entry site (ires), E. coli LacZ gene, Rabbit beta-globin polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP gene, Mouse hprt splice donor, mouse Pgk promoter*, Mouse synaptonemal protein 1 promoter*, Human growth hormone polyadenylation signal*, pBluescript*[E. coli neomycin resistance gene was not detected by PCR. Detection of introduced genes (asterisk) were not tested.]
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data