Strain Data Sheet

RBRC01550

Strain Information

Image
BRC No.RBRC01550
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Syt5<Tn(sb-lacZ,GFP)OR153Jtak>/JtakRbrc
Former Common nameGene trap of Syt5 (synaptotagmin V), OR153
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system.
Keng V W, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins N A, Copeland N G, Horie K, Takeda J
Nat. Methods, 2, 763-769 (2005). 16179923

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Horie K, Yusa K, Yae K, Odajima J, Fischer S E, Keng V W, Hayakawa T, Mizuno S, Kondoh G, Ijiri T, Matsuda Y, Plasterk R H, Takeda J
Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
mouse synaptonemal protein 1 promoter7
BCL2Splice acceptor (human bcl-2)7BCL2
GFPGreen Fluorescent Protein (Aequorea victoria)7CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
GHGrowth hormone polyA (human)7GH
HBB2rabbit beta-globin polyA7HBB2
IRESinternal ribosomal entry site (EMCV)7
SBSleeping Beauty transposon7SB
SBSleeping Beauty transposase7SB
Splice donorSplice donor (mouse hprt)7Splice donor
Syt5synaptotagmin V7Syt5transposon insertion OR153, Junji Takeda
lacZbeta-galactosidase (E. coli)7

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNASleeping Beauty トランスポゾン, Sleeping Beauty トランスポゼース, GFP 遺伝子, LacZ 遺伝子, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires),Human bcl-2 スプライスアクセプター, Mouse hprt スプライスドナー, Cytomegalovirus エンハンサー, Chicken beta-actin プロモーター, Rabbit beta-globin ポリA付加シグナル, Mouse synaptonemal protein 1 プロモーター, Human growth hormone ポリ A付加シグナル, pBluescript
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
    DepositorJunji Takeda (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Takeda Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data