Strain Data Sheet



BRC No.RBRC01544
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Sirpa<tm1Nog>/Rbrc
Former Common nameSHPS-1 Knockout mice
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NJcl
Strain developmentDeveloped by Dr. Kenjiro Inagaki, Kobe University School of Medicine in 2000. The construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed C57BL/6N over 10 times.
Strain descriptionSHPS-1 knockout mice. Exon 7 and 8 were replaced with a nemoycin selection cassette. SHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene ia a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile.
ReferencesJ. Biol. Chem., 277 (42) 39833-9 (2002). 12167615
EMBO J., 19, 6721-6731 (2000). 11118207

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sirpasignal-regulatory protein alpha2Sirpa<tm1Nog>targeted mutation 1, Tetsuya NoguchiBit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sirpa genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must submit its receipt to the DEPOSITOR immediately after receiving the BIOLOGICAL RESOURCE.
DepositorTakashi Matozaki (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genetic Background
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

Mark Kearns, William J. Janssen, and Peter M. Henson
Signal Inhibitory Regulatory Protein Alpha Influences Macrophage Clearance From The Alveolar Space After An Inflammatory Stimulus.
Am. J. Respir. Crit. Care Med. 181 A2761(2010)

Cham LB, Torrez Dulgeroff LB, Tal MC, Adomati T, Li F, Bhat H, Huang A, Lang PA, Moreno ME, Rivera JM, Galkina SA, Kosikova G, Stoddart CA, McCune JM, Myers LM, Weissman IL, Lang KS, Hasenkrug KJ.
Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection.
Cell Rep 31(2) 107494(2020) 32294445

Li LX, Atif SM, Schmiel SE, Lee SJ, McSorley SJ.
Increased susceptibility to Salmonella infection in signal regulatory protein α-deficient mice.
J Immunol 189(5) 2537-44(2012) 22851710

Wang L, Lu Y, Deng S, Zhang Y, Yang L, Guan Y, Matozaki T, Ohnishi H, Jiang H, Li H.
SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress.
J Neurochem 122(4) 834-43(2012) 22671569

Sarute N, Cheng H, Yan Z, Salas-Briceno K, Richner J, Rong L, Ross SR.
Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways.
PLoS Pathog 17(6) e1009662(2021) 34097709