Strain Data Sheet
RBRC01544
Information1 | |
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| Image |  |
| BRC No. | RBRC01544 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Sirpa<tm1Nog>/Rbrc |
| Former Common name | SHPS-1 Knockout mice |
| H-2 Haplotype | |
| ES Cell line | E14.1 [129P2/OlaHsd] |
| Background strain | C57BL/6NJcl |
| Appearance | black |
| Strain development | Developed by Dr. Kenjiro Inagaki, Kobe University School of Medicine in 2000. The construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed C57BL/6N over 10 times. |
| Strain description | SHPS-1 knockout mice. Exon 7 and 8 were replaced with a nemoycin selection cassette. SHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene ia a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile. |
| References | J. Biol. Chem., 277 (42) 39833-9 (2002). 12167615 EMBO J., 19, 6721-6731 (2000). 11118207 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sirpasignal-regulatory protein alpha2Sirpa<tm1Nog>targeted mutation 1, Tetsuya NoguchiBit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sirpa genomic DNA |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must submit its receipt to the DEPOSITOR immediately after receiving the BIOLOGICAL RESOURCE. |
| Depositor | Takashi Matozaki (Gunma University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genetic Background Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Mark Kearns, William J. Janssen, and Peter M. Henson Signal Inhibitory Regulatory Protein Alpha Influences Macrophage Clearance From The Alveolar Space After An Inflammatory Stimulus. Am. J. Respir. Crit. Care Med. 181 A2761(2010) Cham LB, Torrez Dulgeroff LB, Tal MC, Adomati T, Li F, Bhat H, Huang A, Lang PA, Moreno ME, Rivera JM, Galkina SA, Kosikova G, Stoddart CA, McCune JM, Myers LM, Weissman IL, Lang KS, Hasenkrug KJ. Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection. Cell Rep 31(2) 107494(2020) 32294445 Li LX, Atif SM, Schmiel SE, Lee SJ, McSorley SJ. Increased susceptibility to Salmonella infection in signal regulatory protein α-deficient mice. J Immunol 189(5) 2537-44(2012) 22851710 Wang L, Lu Y, Deng S, Zhang Y, Yang L, Guan Y, Matozaki T, Ohnishi H, Jiang H, Li H. SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress. J Neurochem 122(4) 834-43(2012) 22671569 Sarute N, Cheng H, Yan Z, Salas-Briceno K, Richner J, Rong L, Ross SR. Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways. PLoS Pathog 17(6) e1009662(2021) 34097709 |