RBRC01515

Information1

Image
BRC No.RBRC01515
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(SLC18A3-cre)KMisa/MisaRbrc
Former Common nameVAChT-Cre.Early, (Formerly VAChT-Cre.Fast)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hidemi Misawa at Tokyo Metropolitan Institute for Neuroscience in 2003. The VaChT-Cre transgene was injected into the pronuclei of fertilized oocytes of C57BL/6 mice.
Strain descriptionTg(SLC18A3-cre)KMisa, RBRC01515, is a transgenic Cre-driver mouse line, in which Cre expression is driven by the 11.3 kb human vesicular acetylcholine transporter (VAChT) promoter (Misawa et al., 2003). The lines showed restricted Cre expression in approximately one half (40-60%) of the postnatal somatic motor neurons and cholinergic neurons of the medial habenular nucleus. Transgenic expression was not observed in visceromotor or other central or peripheral cholinergic neurons. Cre expression began on approximately postnatal day 7 and reached at maximal levels by 5 weeks of age (earlier onset than Tg(SLC18A3-cre)AMisa, RBRC01516). Re-evaluation (Misawa et al., 2016) of the line by using subtype selective markers showed preferred Cre expression in slow-type motor neurons (S- and FR-type) innervating type I or IIa muscle fibers. To avoid confusion the common name of the line was changed from VAChT-Cre.Fast to VAChT-Cre.Early. Ref. Genesis, 54, 568-572 (2016).
Colony maintenancebackcross to C57BL/6 (Hemizygote x C57BL/6NJcl)
ReferencesGenesis, 54, 568-572 (2016). 27596971
Genesis, 37, 44-50 (2003). 14502577

Health Report

Examination Date / Room / Rack2009/08/24Room:3-CRack:D
2009/06/22Room:3-CRack:D
2009/04/21Room:3-CRack:D
2009/04/20Room:3-CRack:D
2009/02/23Room:3-CRack:D
2008/12/15Room:3-CRack:D
2008/10/20Room:3-CRack:D
2008/08/25Room:3-CRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcrehuman vesicular acetylcholine transporter

Information2

Donor DNAHuman vesicular acetylcholine transporter (SLC18A)genomic DNA, Phage P1 Cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish GFP cDNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. A prior agreement for collaboration with the depositor should be required.
DepositorHidemi Misawa (Kyoritsu College of Pharmacy)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data