RBRC01514

Information1

Image
BRC No.RBRC01514
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mknk1<tm1Fuku> Mknk2<tm1Fuku>/FukuRbrc
Former Common nameMnk1 and Mnk2 deficient mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Rikiro Fukunaga, Graduate School of Frontier Biosciences, Osaka University in 2003. A neomycin selection cassette replaced a fragment from 5 to 6 exons of the Mnk1 and a fragment of 5 to 9 exons of the Mnk1, respectively. R1 ES cells were used. The mutant mice are crossed to C57BL/6.
Strain descriptionMnk1 gene knockout mice (RBRC01512), Mnk2 gene knockout mice (RBRC01513), Mnk1 and Mnk2 genes double knockout mice (RBRC01514). Mnk1 and Mnk2 (MAP kinase-interacting serine/threonine kinase 1 and 2) genes are protein kinases that are directly phosphorylated and activated by extracellular signal-regulated kinase or p38 mitogen-activated protein kinases. These genes are essential for constitutive and inducible phosphorylation of eukaryotic initiation factor 4E, but not for cell growth or development. This strain is useful for elucidation of the role of the functions of Mnk1 and Mnk2 genes. Mnk1 homozygous, Mnk2 homozygous and Mnk1/Mnk2 double homozygous knockout mice are viable and fertile.
Colony maintenanceMnk1: Homozygote x Homozygote [or Crossig to C57BL/6NCrlCrlj]; Mnk2: Homozygote x Homozygote [or Crossig to C57BL/6NCrlCrlj]
ReferencesMol. Cell. Biol., 24, 6539-6549 (2004). 15254222

Health Report

Examination Date / Room / Rack2014/03/24Room:4-ARack:H
2014/01/29Room:4-ARack:H
2014/01/20Room:4-ARack:H
2014/01/20Room:4-ARack:H
2014/01/20Room:4-ARack:H
2013/11/25Room:4-ARack:H
2013/09/24Room:4-ARack:H
2013/09/04Room:4-ARack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mknk2MAP kinase-interacting serine/threonine kinase 210Mknk2<tm1Fuku>targeted mutation 1, Rikiro Fukunaga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mknk1MAP kinase-interacting serine/threonine kinase 14Mknk1<tm1Fuku>targeted mutation 1, Rikiro Fukunaga

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse phosphoglycerate kinase-1 promoter(PGK promoter), E. coli neo, mouse Mnk1 genomic DNA, mouse Mnk2 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 24, 6539-6549 (2004).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorRikiro Fukunaga (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Contact Information (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data