Strain Information | |
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Image | |
BRC No. | RBRC01513 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Mknk2<tm1Fuku>/FukuRbrc |
Former Common name | Mnk2-KO, Mnk2 knockout mouse |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Rikiro Fukunaga, Graduate School of Frontier Biosciences, Osaka University in 2003. A neomycin selection cassette replaced a fragment from 5 to 6 exons of the Mnk1 and a fragment of 5 to 9 exons of the Mnk1, respectively. R1 ES cells were used. The mutant mice are crossed to C57BL/6. |
Strain description | Mnk1 gene knockout mice (RBRC01512), Mnk2 gene knockout mice (RBRC01513), Mnk1 and Mnk2 genes double knockout mice (RBRC01514). Mnk1 and Mnk2 (MAP kinase-interacting serine/threonine kinase 1 and 2) genes are protein kinases that are directly phosphorylated and activated by extracellular signal-regulated kinase or p38 mitogen-activated protein kinases. These genes are essential for constitutive and inducible phosphorylation of eukaryotic initiation factor 4E, but not for cell growth or development. This strain is useful for elucidation of the role of the functions of Mnk1 and Mnk2 genes. Mnk1 homozygous, Mnk2 homozygous and Mnk1/Mnk2 double homozygous knockout mice are viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] |
References | Mnk2 and Mnk1 are essential for constitutive and inducible phosphorylation of eukaryotic initiation factor 4E but not for cell growth or development. Ueda T, Watanabe-Fukunaga R, Fukuyama H, Nagata S, Fukunaga R Mol. Cell. Biol., 24, 6539-6549 (2004). 15254222 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Mknk2 | MAP kinase-interacting serine/threonine kinase 2 | 10 | Mknk2 | targeted mutation 1, Rikiro Fukunaga | |||
neo | neomycin resistance gene (E. coli) | 10 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Mouse phosphoglycerate kinase-1 promoter(PGK promoter), E. coli neo, mouse Mnk2 genomic DNA |
Research application | Cell Biology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 24, 6539-6549 (2004). RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Rikiro Fukunaga (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |