Strain Data Sheet

RBRC01507

Strain Information

Image
BRC No.RBRC01507
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Olig2<tm1(cre/Esr1*)Htak>/Rbrc
Former Common nameOlig2-CreER knock in mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hirohide Takebayashi at Kyoto Universtiy in 2002. A cassette containing neo and tamoxifen-inducible cre genes was inserted into the exon 2 encoding the basic helix-loop-helix domain. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionB6.Cg-Olig2<tm1(cre/Esr1)Htak>. These knock-in mice have a tamoxifen-inducible cre (cre-ER<TM>) at the Olig2 locus. Cre-ER<TM> is expressed in motoneuron and oligodendrocyte progenitors.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrj)Homozygous mutant mice die postnatally.
References
The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages.
Takebayashi H, Nabeshima Y, Yoshida S, Chisaka O, Ikenaka K, Nabeshima Y
Current Biology, 12, 1157-1163 (2002). 12121626

Olig2-positive progenitors in the embryonic spinal cord give rise not only to motoneurons and oligodendrocytes, but also to a subset of astrocytes and ependymal cells.
Masahira N, Takebayashi H, Ono K, Watanabe K, Ding L, Furusho M, Ogawa Y, Nabeshima Y, Alvarez-Buylla A, Shimizu K, Ikenaka K
Dev. Biol., 293, 358-369 (2006). 16581057

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Esr1*estrogen receptor 1 (alpha), mouse ER with a mutation G525R)16Esr1*
Olig2oligodendrocyte transcription factor 216Olig2targeted mutation 1, Hirohide Takebayashi
crePhage P1 Cre recombinase16cre
neoneomycin resistance gene (E. coli)16herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell differentiation(MP:0005076)

  • abnormal dorsal root ganglion morphology(MP:0000961)

  • abnormal neuronal migration(MP:0006009)

  • abnormal posture(MP:0001504)

  • abnormal proprioceptive neuron morphology(MP:0004297)
  • more 11 phenotypes
  • abnormal reflex(MP:0001961)

  • abnormal sensory neuron innervation pattern(MP:0000968)

  • abnormal sensory neuron morphology(MP:0000965)

  • abnormal spinal cord morphology(MP:0000955)

  • abnormal spinal cord ventral horn morphology(MP:0005112)

  • aphagia(MP:0001438)

  • decreased motor neuron number(MP:0000939)

  • decreased oligodendrocyte progenitor number(MP:0000954)

  • hypotonia(MP:0004144)

  • neonatal lethality, complete penetrance(MP:0011087)

  • nervous system phenotype(MP:0003631)
  • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 Cre recombinase, mouse estrogen receptor cDNA (Nucleic Acid Res. (1995) 23, 1686), FLAG epitope, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Olig2 genomic DNA
    Research applicationCell Biology Research
    Cre/loxP system
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Current Biology, 12, 1157-1163 (2002).The RECIPIENT should contact the DEPOSITOR in the case of application for patent or commercial use with the results from the use of the BIOLOGICAL RESOURCE. Within the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC, the RECIPIENT should consult DEPOSITOR in regard to co-authorship of the DEPOSITOR/DEVELOPER in publication(s) resulted by the use of the BIOLOGICAL RESOURCE.
    DepositorHirohide Takebayashi (National Institute for Physiological Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month July 2006

    BRC mice in Publications

    No Data