BRC No.RBRC01507
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Olig2<tm1(cre/Esr1*)Htak>/Rbrc
Former Common nameOlig2-CreER knock in mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hirohide Takebayashi at Kyoto Universtiy in 2002. A cassette containing neo and tamoxifen-inducible cre genes was inserted into the exon 2 encoding the basic helix-loop-helix domain. The mutant mice were backcrossed to C57BL/6N.
Strain descriptionB6.Cg-Olig2<tm1(cre/Esr1)Htak>. These knock-in mice have a tamoxifen-inducible cre (cre-ER<TM>) at the Olig2 locus. Cre-ER<TM> is expressed in motoneuron and oligodendrocyte progenitors.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrj)Homozygous mutant mice die postnatally.
ReferencesCurrent Biology, 12, 1157-1163 (2002). 12121626
Dev. Biol., 293, 358-369 (2006). 16581057

Health Report

Examination Date / Room / Rack2009/03/16Room:4-ARack:H


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Olig2oligodendrocyte transcription factor 216Olig2<tm1(cre/Esr1)Htak>targeted mutation 1, Hirohide Takebayashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Esrestrogen receptor 1 (ER)16Esr

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase16cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)16herpes simplex virus thymidine kinase promoter (HSV tk promoter)


Donor DNAPhage P1 Cre recombinase, mouse estrogen receptor cDNA(Nucleic Acid Res. (1995) 23, 1686), FLAG epitope, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Olig2 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Current Biology, 12, 1157-1163 (2002).The RECIPIENT should contact the DEPOSITOR in the case of application for patent or commercial use with the results from the use of the BIOLOGICAL RESOURCE. Within the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC, the RECIPIENT should consult DEPOSITOR in regard to co-authorship of the DEPOSITOR/DEVELOPER in publication(s) resulted by the use of the BIOLOGICAL RESOURCE.
DepositorHirohide Takebayashi (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month July 2006
Breeding characters

BRC mice in Publications

No Data