BRC No.RBRC01491
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Lrrn4<tm1>/Rbrc
Former Common nameNLRR4-KO(B6); B6.Cg-B430119L13Rik<tm>
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Takayoshi Bando and Atsushi Miyajima at the University of Tokyo in 2001. The TT2 ES cells originated from an F1 embryo between C57BL/6 and CBA mice were used. The targeting construct contains relpaced exons with the beta-galactosidase and the neomycin resistance genes by homologous reconbination. The NLRR4 deficient mice were backcrossed to C57BL/6JJcl.
Strain descriptionB6.Cg-B430119L13Rik<tm>. NLRR4 is a member of the neuronal leucine-rich repeat protein family. NLRR proteins are type I transmembrane proteins with leucine-rich repeats and a fibronectine type III repeat, and expressed in neuronal tissues. NLRR4 deficient mice have a severe damage to their long-term memory in hippocampus-dependent tasks, while their hippocampal long-term potentiation was intact. NLRR4 plays a role in hippocamus-dependent long-lasting memory.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6J)Homozygous deficient mice are viable and fertile.
ReferencesMol. Cell. Biol., 25, 4166-4175 (2005). 15870286

Health Report

Examination Date / Room / RackNo Data


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Lrrn4leucine rich repeat neuronal 42Lrrn4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), poly A, E. coli neo, E. coli lacZ, mouse Lrrn4 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 10, 4160-4175 (2005).The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
DepositorAtsushi Miyajima (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data