RBRC01477

Information1

Image
BRC No.RBRC01477
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Ptprj<tm1Taka>/SataRbrc
Former Common nameCD148/DEP-1 KO/PTPRJ, CD148 KO
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr Takamune Takahashi at Vanderbilt University Medical Center in 2003. A targeting construct containing EGFP and neo was transfered into ES cells to disrupt Ptprj gene. GFP fluorescence was not detected in mutant mice.
Strain descriptionB6.129S6-Ptprj<tm1Taka>. Protein tyrosine phospatase, receptor type J (CD148) is a member of the receptor tyrosine phosphatase family playing an important role in cell growth, differentiation, and development. Homozygous Ptprj<tm1Taka> mutant mice were embryonic lethal due to abnormal cardiovascular system morphology.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesMol. Cell. Biol., 23, 1817-1831 (2003). 12588999

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ptprjprotein tyrosine phosphatase, receptor type, J2Ptprj<tm1Taka>targeted mutation 1, Takamune Takahashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), Jellyfish GFP cDNA, Phage P1 loxP, E. coli neo, mouse CD148 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 23, 1817-1831 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasanobu Satake (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data