Strain Data Sheet


Strain Information

BRC No.RBRC01474
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Fas<tm1Osa>/OsaRbrc
Former Common nameB6 Fas-KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Masashi Adachi and Shigekazu Nagata at Osaka University Medical School in 1995.
Strain descriptionFas is a cell surface receptor belonging to the tumor necrosis factor receptor superfamily which transduces apoptotic signals in many cell types. Fas deficient mutant mice show lymphoma and autoimmune disorders. In a few month after birth, the mice develop lymphadenoma and splenomegaly by an accumulation of abnormal polyclonal T cells with the CD4-CD8- double negative.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)Homozygous mutant mice show lymphoma and autoimmune disorders. 50% dead at about 5 months of age.
ReferencesNat. Genet., 11(3), 294-300 (1995). 7581453

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FasFas (TNF receptor superfamily member)19Fas<tm1Osa>targeted mutation 1, Osaka University Medical SchoolAPO-1, CD95, TNFR6, Tnfrsf6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)19

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)19neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAE. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Fas genomic DNA
Research applicationApoptosis Research
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Genetics, 11, 294-300 (1995).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Jeong D, Kim HS, Kim HY, Kang MJ, Jung H, Oh Y, Kim D, Koh J, Cho SY, Jeon YK, Lee EB, Lee SH, Shin EC, Kim HM, Yi EC, Chung DH.
Soluble Fas ligand drives autoantibody-induced arthritis by binding to DR5/TRAIL-R2.
Elife 10 (2021) 34223817