RBRC01458

Information1

Image
BRC No.RBRC01458
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Dpt<tm1Hsh>/NakaRbrc
Former Common nameDermatopontin KO mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hroshi Shinkai, Chiba University at 2002. A pgk-neo was transfered into ES cells to relpace exon 1 of Dpt gene.
Strain descriptionDermatopontin (Dpt) is a collagen-associated protein, is widely distributed in extracellular matrix. Homozygote mutant mice not exhibit any obvious anatomical abnormality, but the skin elasticity was increased. This strain is useful for study of the mechanism and the process of collagen fibrillogenesis.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)Homozygote mutant mice are fertile.
ReferencesJ. Invest. Dermatol., 119, 678-683 (2002). 12230512

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
DptDermatopontin1Dpt<tm1Hsh>targeted mutation 1, Hiroshi Shinkai

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Dpt genomic DNA
Research applicationCell Biology Research
Dermatology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Invest. Dermatol., 119, 678-683 (2002).
DepositorToshinori Nakayama (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data