Strain Data Sheet

RBRC01456

Information1

Image
BRC No.RBRC01456
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Neurog3-cre)24Syos
Former Common namengn3/Cre transgenic mouse, ngn3/Cre Tg#24
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Shosei Yoshida, Graduate School of Medicine, Kyoto University in 2001. The ngn3-Cre transgene containing a 23-kb genomic region of the mouse ngn3 locus and cre recombinase gene was injected into the pronuclei of B6D2F1 fertilized eggs. The transgenic mice were backcrossed to C57BL/6.
Strain descriptionThese transgenic mice express a cre recombinase gene under the control of the mouse Neurogenin 3 gene promoter. This can be used to make an conditional knock-out by crossing with the other genetically-engineered mice that have floxed genes. The expression of cre recombinase was observed in positive spermatogonia, pancreatic/enteric endocrine precursors.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrSlc]. Tg mice are viable and fertile.
ReferencesDev. Biol., 269, 447-458 (2004). 15110712

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcremouse neurogenin 3 promoter, mouse metallothionein poly A

Information2

Donor DNAmouse ngn3 genomic DNA, phage P1 Cre recombinase, mouse metallothionein gene poly(A) (from pBS185)
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 269, 447-458 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of the BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. This consent shall include the outline of the experiments in order to avoid possible conflict with the DEPOSITOR and other-workers. The RECIPIENT is prohibited to release the BIOLOGICAL RESOURCE to third parties without permission by the DEPOSITOR or use it for Experiments without prior agreement with the DEPOSITOR.
DepositorShosei Yoshida (National Institute for Basic Biology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month July 2006
Genetic Background
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data