RBRC01450

Information1

Image
BRC No.RBRC01450
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Runx1<tm1Sata>/SataRbrc
Former Common nameAML1 KO, Runx1 KO mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Masanobu Satake, Tohoku University in 1998. A targeting vector containing pgk-neo cassette was transfered into ES cells to relpace exons 4.
Strain descriptionB6.129S4-Runx1<tm1Sata>. Runx1, runt related transcription factor 1 (also called AML1) encodes the DNA binding subunit of the heterodimeric transcription factor. Runx1 is one of the most frequent targets of chromosome translocation associated with several subtypes of human acute myeloid or lymphoblastic leukemia. Mice homozygous for the Runx1 null mutantion die embryonic day 12.5 due to massive hemorrhage in the central nervous system. In fetal livers of mutant embryos, no hematopoieteic cells are generated.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesOncogene, 17, 2287-2293 (1998). 9811459

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Runx1runt related transcription factor 116Runx1<tm1Sata>targeted mutation 1, Masanobu Satake

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)16mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Runx1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
Research applicationCancer Research
Cell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 17, 2287-2293 (1998).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use
of it from the DEPOSITOR to avoid possible conflicts with DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a
citation of the following literature designated by the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE,
an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications
resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasanobu Satake (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data