RBRC01400

Information1

Image
BRC No.RBRC01400
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(CAG-Ptafr)1Tksh/TkshRbrc
Former Common namePAFR-Tg, Platelet-activating factor (PAF) receptor transgenic mouse
H-2 Haplotype
ES Cell line
Background strainCrlj:B6D2F1
Appearance
Strain developmentDeveloped by Dr. Satoshi Ishii at the University of Tokyo in 1997. A transgenic construct containing a guinea-pig PAFR gene under the control of the a chiken beta actin promotor and the CMV immediate early enhancer, was introduced into the pronuclei of fertilized eggs of B6D2F2. The transgenic mice were mated with B6D2F1.
Strain descriptionPlatelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. This transgenic mice showed a bronchial hyperreactivity, increased endotoxin lethality and melanocytic tumorigenesis. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo.
Colony maintenanceCross to B6D2F1 (Hemizygote x Crj:B6D2F1)
ReferencesEMBO J., 16, 133-142 (1997). 9009274

Health Report

Examination Date / Room / Rack2007/07/02Room:2-検Rack:D
2007/05/08Room:2-検Rack:D
2007/05/07Room:2-検Rack:D
2007/03/05Room:2-検Rack:D
2007/01/09Room:2-検Rack:D
2006/10/30Room:2-検Rack:D
2006/08/29Room:2-検Rack:D
2006/08/28Room:2-検Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ptafrplatelet-activating factor receptor (guinea-pig)UNPtafrCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), guinea-pig Platelet-activating factor (PAF) receptor cDNA,
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 16, 133-142 (1997).
DepositorTakao Shimizu (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP (Japanese)

BRC mice in Publications

No Data