RBRC01389

Information1

Image
BRC No.RBRC01389
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Ahr<tm1(AHR)Mym>/MymRbrc
Former Common nameHuman AhR knock in mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Takashi Moriguchi et al. in 2001. Human AHR cDNA and neomycin resistant gene were inserted in mouse Ahr gene. This construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. This knock-in mice were backcrossed with C57BL/6J for several times.
Strain descriptionThe aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediates to catalyze xenobiotic Phase I metabolism. Human AhR knock in mouse, possessing human AHR instead of mouse Ahr, is useful as humanized mouse to predict the biological effects of bioaccumulative environmental toxicants like TCDD in humans.
Colony maintenanceBackcross to C57BL/6 (Heterozyote x C57BL/6JJ)
ReferencesProc. Natl. Acad. Sci. USA, 100, 5652-5657 (2003). 12730383

Health Report

Examination Date / Room / Rack2008/03/17Room:4-ARack:I
2008/01/14Room:4-ARack:I
2007/11/12Room:4-ARack:I
2007/09/18Room:4-ARack:I
2007/07/17Room:4-ARack:I
2007/05/22Room:4-ARack:I
2007/05/21Room:4-ARack:I
2007/03/19Room:4-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ahraryl-hydrocarbon receptor12Ahr<tm1(AHR)Mym>targeted mutation 1, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
AHRaryl-hydrocarbon receptor (human)12AHR

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)12herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAhuman arylhydrocarbon receptor AhR cDNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse arylhydrocarbon receptor AhR genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 100, 5652-5657 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayuki Yamamoto (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data