Strain Data Sheet

RBRC01386

Information1

Image
BRC No.RBRC01386
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;FVB-Tg(ACTA1-cre)AMcle/Rbrc
Former Common nameTransgenic mouse, MuCre-A
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe expression construct was created by cloning cDNAs encoding the selected genes and the promoter into a plasmid vector. After verification of the construct by nucleotide sequencing, the transcriptional unit was cleaved out with restriction enzymes, purified with biochemical method and used for microinjection. The offspring were initially examined by PCR genotyping. The in vivo expression was checked by crossing the mice with two Cre-reporter lines.
Strain descriptionMuCreA contains a transcriptional unit consisting of a muscle-specific promoter (alpha-actin promoter) and Cre recombinase gene. The mouse is used to make myogenic-specific knock-outs by crossing with mice that have a floxed gene. The MuCreA produces rapid and early cleavage of floxed genes from all differentiated myogenic cells, including cardiac myocytes and skeletal myotubes.
Colony maintenance
ReferencesInt. J. Biol. Sci., 20;6(6):546-555 (2010). 20877696

Health Report

Examination Date / Room / Rack2022/06/13Room:3-2Rack:D
2022/03/14Room:3-2Rack:D
2021/12/14Room:3-2Rack:D
2021/09/13Room:3-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre<Tg(ACTA1-cre)AMcle>transgene insertion A, Ian S. McLennanhuman alpha actin promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Information2

Donor DNAhuman alpha skeletal muscle actin promoter, phage P1 Cre recombinase, SV40 poly A signal
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. J. Biol. Sci., 20;6(6):546-555 (2010).
DepositorKyoko Koishi (University of Otago)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Azhar M, Wang PY, Frugier T, Koishi K, Deng C, Noakes PG, McLennan IS.
Myocardial deletion of Smad4 using a novel α skeletal muscle actin Cre recombinase transgenic mouse causes misalignment of the cardiac outflow tract.
Int J Biol Sci 6(6) 546-55(2010) 20877696

Fujimoto M, Kitamura H.
Application of the colorimetric loop-mediated isothermal amplification (LAMP) technique for genotyping Cre-driver mice.
J Vet Med Sci (2022) 35228407

Uruno A, Yagishita Y, Katsuoka F, Kitajima Y, Nunomiya A, Nagatomi R, Pi J, Biswal SS, Yamamoto M.
Nrf2-Mediated Regulation of Skeletal Muscle Glycogen Metabolism.
Mol Cell Biol 36(11) 1655-72(2016) 27044864

Uruno A, Furusawa Y, Yagishita Y, Fukutomi T, Muramatsu H, Negishi T, Sugawara A, Kensler TW, Yamamoto M.
The Keap1-Nrf2 system prevents onset of diabetes mellitus.
Mol Cell Biol 33(15) 2996-3010(2013) 23716596