RBRC01385

Information1

Image
BRC No.RBRC01385
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/McleRbrc
Former Common nameTransgenic mouse, PTR-H
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentThe expression construct was created by cloning cDNAs encoding the selected genes and the promoter into a plasmid vector. After verification of the construct by nucleotide sequencing, the transcriptional unit was cleaved out with restriction enzymes, purified with biochemical method and used for microinjection. The offspring were initially examined by PCR genotyping and in vitro expression of the marker, EGFP, after transfection with Tet-OFF expression vector (Clontech). The entire process took from 1999 to 2003.
Strain descriptionThe transcriptional unit integrated into PTR-H consists of a dominant negative form of TGF-beta type II receptor gene and EGFP marker under a bidirectional tetracycline-responsive promoter. By crossing with another transgenic mouse that express tetracycline-inducible transcriptional activator, TGF-beta transduction pathway can be disrupted in a conditional manner.
Colony maintenance
ReferencesGenesis 42, 1-5 (2005). 15828000

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tgfbr2transforming growth factor, beta receptor IIUNTgfbr2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNE. coli bidirectional TRE promoter (tetracycline responsive promoter)

Information2

Donor DNATGF-beta type II receptor, Jellyfish GFP cDNA, E. coli bidirectional tetracycline-responsive promoter
Research applicationFluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Frugier, T., Kyoko, K., Matthaei, K.I. and McLennan, I.S. (2005). Transgenic mice carrying a tetracycline-inducible transforming growth factor beta receptor (T_RII). Genesis 42, 1-5.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKyoko Koishi (University of Otago)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

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