Strain Data Sheet


Strain Information

BRC No.RBRC01361
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Trp53<tm1Sia>/Rbrc
Former Common nameC57BL-p53+/-
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain descriptionTrp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesOncogene, 8, 3313-3322 (1993). 7504233

Health Report

Examination Date / Room / Rack2024/04/22Room:3-CRack:E


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trp53transformation-related protein 5311Trp53<tm1Sia>targeted mutation 1, Shinichi Aizawap53

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA
Research applicationCancer Research
Cell Biology Research
Immunology and Inflammation Research
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 8, 3313-3322 (1993).Please inform Laboratory for Animal Resources and Genetic Engineering, CDB (e mail: about the paper when published.
DepositorOhtsura Niwa (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Jun 2005
Genetic Background

BRC mice in Publications

Kobayashi M, Hoshino S, Abe T, Okita N, Tagawa R, Nagai W, Konno R, Suzuki Y, Furuya K, Ishikawa N, Okado H, Oku M, Iwamoto M, Miura Y, Sudo Y, Higami Y.
Identification of WWP1 as an obesity-associated E3 ubiquitin ligase with a protective role against oxidative stress in adipocytes.
Biochem Biophys Res Commun 508(1) 117-122(2019) 30471861

Saito Y, Onishi N, Takami H, Seishima R, Inoue H, Hirata Y, Kameyama K, Tsuchihashi K, Sugihara E, Uchino S, Ito K, Kawakubo H, Takeuchi H, Kitagawa Y, Saya H, Nagano O.
Development of a functional thyroid model based on an organoid culture system.
Biochem Biophys Res Commun 497(2) 783-789(2018) 29470983

Okita N, Ishikawa N, Mizunoe Y, Oku M, Nagai W, Suzuki Y, Matsushima S, Mikami K, Okado H, Sasaki T, Higami Y.
Inhibitory effect of p53 on mitochondrial content and function during adipogenesis.
Biochem Biophys Res Commun 446(1) 91-7(2014) 24565844

Mai HN, Sharma G, Sharma N, Shin EJ, Kim DJ, Pham DT, Trinh QD, Jang CG, Nah SY, Jeong JH, Kim HC.
Genetic depletion of p53 attenuates cocaine-induced hepatotoxicity in mice.
Biochimie 158 53-61(2019) 30576773

Yamauchi H, Katayama K, Ueno M, Kanemitsu H, Nam C, Mikami T, Saito A, Ishida Y, Uetsuka K, Doi K, Ohmach Y, Nakayama H.
Etoposide induces TRP53-dependent apoptosis and TRP53-independent cell cycle arrest in trophoblasts of the developing mouse placenta.
Biol Reprod 80(4) 813-22(2009) 19109225

Cai W, Fujita T, Hidaka Y, Jin H, Suita K, Shigeta M, Kiyonari H, Umemura M, Yokoyama U, Sadoshima J, Ishikawa Y.
Translationally controlled tumor protein (TCTP) plays a pivotal role in cardiomyocyte survival through a Bnip3-dependent mechanism.
Cell Death Dis 10(8) 549(2019) 31320615

Kobatake K, Ikeda KI, Nakata Y, Yamasaki N, Ueda T, Kanai A, Sentani K, Sera Y, Hayashi T, Koizumi M, Miyakawa Y, Inaba T, Sotomaru Y, Kaminuma O, Ichinohe T, Honda ZI, Yasui W, Horie S, Black PC, Matsubara A, Honda H.
Kdm6a Deficiency Activates Inflammatory Pathways, Promotes M2 Macrophage Polarization, and Causes Bladder Cancer in Cooperation with p53 Dysfunction.
Clin Cancer Res 26(8) 2065-2079(2020) 32047002

Sasaki H, Sugiyama M, Sasaki N.
Establishment of renal proximal tubule cell lines derived from the kidney of p53 knockout mice.
Cytotechnology 71(1) 45-56(2019) 30603921

Tanikawa C, Zhang YZ, Yamamoto R, Tsuda Y, Tanaka M, Funauchi Y, Mori J, Imoto S, Yamaguchi R, Nakamura Y, Miyano S, Nakagawa H, Matsuda K.
The Transcriptional Landscape of p53 Signalling Pathway.
EBioMedicine 20 109-119(2017) 28558959

Liu X, Sato N, Yabushita T, Li J, Jia Y, Tamura M, Asada S, Fujino T, Fukushima T, Yonezawa T, Tanaka Y, Fukuyama T, Tsuchiya A, Shikata S, Iwamura H, Kinouchi C, Komatsu K, Yamasaki S, Shibata T, Sasaki AT, Schibler J, Wunderlich M, O'Brien E, Mizukawa B, Mulloy JC, Sugiura Y, Takizawa H, Shibata T, Miyake K, Kitamura T, Goyama S.
IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia.
EMBO Mol Med 15(1) e15631(2023) 36453131

Tochitani T, Kanemitsu H, Yamauchi H, Uchida K, Nakayama H.
5-azacytidine, a chemotherapeutic drug, induces TRAIL-mediated apoptosis in mouse thymocytes in vivo.
Exp Toxicol Pathol 63(3) 237-42(2011) 20092982

Nam C, Doi K, Nakayama H.
Etoposide induces G2/M arrest and apoptosis in neural progenitor cells via DNA damage and an ATM/p53-related pathway.
Histol Histopathol 25(4) 485-93(2010) 20183801

Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Kotani N, Honke K, Suzumura A, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K.
Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase.
J Biol Chem 290(26) 16043-58(2015) 25940087

Tobisawa Y, Imai Y, Fukuda M, Kawashima H.
Sulfation of colonic mucins by N-acetylglucosamine 6-O-sulfotransferase-2 and its protective function in experimental colitis in mice.
J Biol Chem 285(9) 6750-60(2010) 20018871

Okahashi N, Kohno S, Kitajima S, Matsuda F, Takahashi C, Shimizu H.
Metabolic characterization of cultured mammalian cells by mass balance analysis, tracer labeling experiments and computer-aided simulations.
J Biosci Bioeng 120(6) 725-31(2015) 25936961

Nagashima T, Ninomiya T, Nakamura Y, Nishimura S, Ohashi A, Aoki J, Mizoguchi T, Tonogi M, Takahashi T.
p53 deficiency promotes bone regeneration by functional regulation of mesenchymal stromal cells and osteoblasts.
J Bone Miner Metab 40(3) 434-447(2022) 35195777

Kawashima H.
Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues.
Methods Enzymol 479 243-56(2010) 20816170

Shin EJ, Nam Y, Lee JW, Nguyen PT, Yoo JE, Tran TV, Jeong JH, Jang CG, Oh YJ, Youdim MBH, Lee PH, Nabeshima T, Kim HC.
N-Methyl, N-propynyl-2-phenylethylamine (MPPE), a Selegiline Analog, Attenuates MPTP-induced Dopaminergic Toxicity with Guaranteed Behavioral Safety: Involvement of Inhibitions of Mitochondrial Oxidative Burdens and p53 Gene-elicited Pro-apoptotic Change.
Mol Neurobiol 53(9) 6251-6269(2016) 26563498

Zhang P, Ohkawa Y, Yamamoto S, Momota H, Kato A, Kaneko K, Natsume A, Farhana Y, Ohmi Y, Okajima T, Bhuiyan RH, Wakabayashi T, Furukawa K, Furukawa K.
St8sia1-deficiency in mice alters tumor environments of gliomas, leading to reduced disease severity.
Nagoya J Med Sci 83(3) 535-549(2021) 34552288

Saito Y, Chikenji TS, Matsumura T, Nakano M, Fujimiya M.
Exercise enhances skeletal muscle regeneration by promoting senescence in fibro-adipogenic progenitors.
Nat Commun 11(1) 889(2020) 32060352

Hayashi Y, Goyama S, Liu X, Tamura M, Asada S, Tanaka Y, Fukuyama T, Wunderlich M, O'Brien E, Mizukawa B, Yamazaki S, Matsumoto A, Yamasaki S, Shibata T, Matsuda K, Sashida G, Takizawa H, Kitamura T.
Antitumor immunity augments the therapeutic effects of p53 activation on acute myeloid leukemia.
Nat Commun 10(1) 4869(2019) 31653912

Kanemitsu H, Yamauchi H, Komatsu M, Yamamoto S, Okazaki S, Uchida K, Nakayama H.
6-mercaptopurine (6-MP) induces p53-mediated apoptosis of neural progenitor cells in the developing fetal rodent brain.
Neurotoxicol Teratol 31(4) 198-202(2009) 19281843

Tokumasu R, Yasuhara R, Kang S, Funatsu T, Mishima K.
Transcription factor FoxO1 regulates myoepithelial cell diversity and growth.
Sci Rep 14(1) 1069(2024) 38212454

Tamura M, Yonezawa T, Liu X, Asada S, Hayashi Y, Fukuyama T, Tanaka Y, Kitamura T, Goyama S.
Opposing effects of acute versus chronic inhibition of p53 on decitabine's efficacy in myeloid neoplasms.
Sci Rep 9(1) 8171(2019) 31160638

Kanatsu-Shinohara M, Naoki H, Tanaka T, Tatehana M, Kikkawa T, Osumi N, Shinohara T.
Regulation of male germline transmission patterns by the Trp53-Cdkn1a pathway.
Stem Cell Reports 17(9) 1924-1941(2022) 35931081

Kitajima S, Kohno S, Kondoh A, Sasaki N, Nishimoto Y, Li F, Abdallah Mohammed MS, Muranaka H, Nagatani N, Suzuki M, Kido Y, Takahashi C.
Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts.
Stem Cells 33(5) 1657-69(2015) 25694388