RBRC01361

Information1

Image
BRC No.RBRC01361
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Trp53<tm1Sia>/Rbrc
Former Common nameC57BL-p53+/-
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain descriptionTrp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6NCrSlc]
ReferencesOncogene, 8, 3313-3322 (1993). 7504233

Health Report

Examination Date / Room / Rack2020/02/17Room:3-CRack:E
2019/12/16Room:3-CRack:E
2019/10/21Room:3-CRack:E
2019/08/26Room:3-CRack:E
2019/07/08Room:3-CRack:E
2019/07/08Room:3-CRack:E
2019/06/17Room:3-CRack:E
2019/04/15Room:3-CRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Trp53transformation-related protein 5311Trp53<tm1Sia>targeted mutation 1, Shinichi Aizawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA
Research applicationCancer Research
Cell Biology Research
Immunology and Inflammation Research
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 8, 3313-3322 (1993).Please inform Laboratory for Animal Resources and Genetic Engineering,
CDB (e mail: mutant.bdr@riken.jp) about the paper when published.
DepositorOhtsura Niwa (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Jun 2005
Genetic Background
Breeding characters

BRC mice in Publications

Saito Y, Onishi N, Takami H, Seishima R, Inoue H, Hirata Y, Kameyama K, Tsuchihashi K, Sugihara E, Uchino S, Ito K, Kawakubo H, Takeuchi H, Kitagawa Y, Saya H, Nagano O.
Development of a functional thyroid model based on an organoid culture system.
Biochem. Biophys. Res. Commun. (2018) 29470983

Kobayashi M, Hoshino S, Abe T, Okita N, Tagawa R, Nagai W, Konno R, Suzuki Y, Furuya K, Ishikawa N, Okado H, Oku M, Iwamoto M, Miura Y, Sudo Y, Higami Y.
Identification of WWP1 as an obesity-associated E3 ubiquitin ligase with a protective role against oxidative stress in adipocytes.
Biochem. Biophys. Res. Commun. (2018) 30471861

Okita N, Ishikawa N, Mizunoe Y, Oku M, Nagai W, Suzuki Y, Matsushima S, Mikami K, Okado H, Sasaki T, Higami Y.
Inhibitory effect of p53 on mitochondrial content and function during adipogenesis.
Biochem. Biophys. Res. Commun. (2014) 24565844

Mai HN, Sharma G, Sharma N, Shin EJ, Kim DJ, Pham DT, Trinh QD, Jang CG, Nah SY, Jeong JH, Kim HC.
Genetic depletion of p53 attenuates cocaine-induced hepatotoxicity in mice.
Biochimie (2018) 30576773

Yamauchi H, Katayama K, Ueno M, Kanemitsu H, Nam C, Mikami T, Saito A, Ishida Y, Uetsuka K, Doi K, Ohmach Y, Nakayama H.
Etoposide induces TRP53-dependent apoptosis and TRP53-independent cell cycle arrest in trophoblasts of the developing mouse placenta.
Biol. Reprod. 80(4) 813-22(2009) 19109225

Sasaki H, Sugiyama M, Sasaki N.
Establishment of renal proximal tubule cell lines derived from the kidney of p53 knockout mice.
Cytotechnology (2019) 30603921

Tanikawa C, Zhang YZ, Yamamoto R, Tsuda Y, Tanaka M, Funauchi Y, Mori J, Imoto S, Yamaguchi R, Nakamura Y, Miyano S, Nakagawa H, Matsuda K.
The Transcriptional Landscape of p53 Signalling Pathway.
EBioMedicine 20 109-119(2017) 28558959

Tochitani T, Kanemitsu H, Yamauchi H, Uchida K, Nakayama H.
5-azacytidine, a chemotherapeutic drug, induces TRAIL-mediated apoptosis in mouse thymocytes in vivo.
Exp. Toxicol. Pathol. (2011) 20092982

Nam C, Doi K, Nakayama H.
Etoposide induces G2/M arrest and apoptosis in neural progenitor cells via DNA damage and an ATM/p53-related pathway.
Histol. Histopathol. 25(4) 485-93(2010) 20183801

Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Kotani N, Honke K, Suzumura A, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K.
Ganglioside GD3 enhances invasiveness of gliomas by forming a complex with platelet-derived growth factor receptor alpha and Yes.
J. Biol. Chem. (2015) 25940087

Tobisawa Y, Imai Y, Fukuda M, Kawashima H.
Sulfation of colonic mucins by N-acetylglucosamine 6-O-sulfotransferase-2 and its protective function in experimental colitis in mice.
J. Biol. Chem. 285(9) 6750-60(2010) 20018871

Okahashi N, Kohno S, Kitajima S, Matsuda F, Takahashi C, Shimizu H.
Metabolic characterization of cultured mammalian cells by mass balance analysis, tracer labeling experiments and computer-aided simulations.
J. Biosci. Bioeng. (2015) 25936961

Kawashima H.
Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues.
Meth. Enzymol. 479 243-56(2010) 20816170

Shin EJ, Nam Y, Lee JW, Nguyen PT, Yoo JE, Tran TV, Jeong JH, Jang CG, Oh YJ, Youdim MB, Lee PH, Nabeshima T, Kim HC.
N-Methyl, N-propynyl-2-phenylethylamine (MPPE), a Selegiline Analog, Attenuates MPTP-induced Dopaminergic Toxicity with Guaranteed Behavioral Safety: Involvement of Inhibitions of Mitochondrial Oxidative Burdens and p53 Gene-elicited Pro-apoptotic Change.
Mol. Neurobiol. (2015) 26563498

Kanemitsu H, Yamauchi H, Komatsu M, Yamamoto S, Okazaki S, Uchida K, Nakayama H.
6-mercaptopurine (6-MP) induces p53-mediated apoptosis of neural progenitor cells in the developing fetal rodent brain.
Neurotoxicol Teratol 31(4) 198-202(2009) 19281843

Kitajima S1, Kohno S, Kondoh A, Sasaki N, Nishimoto Y, Li F, Mohammed MS, Muranaka H, Nagatani N, Suzuki M, Kido Y, Takahashi C
Undifferentiated State Induced by Rb-P53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts
Stem Cells (2015) 25694388