|Former Common name||C57BL-p53+/-|
|ES Cell line||TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]|
|Appearance||black [a/a B/B C/C]|
|Strain development||Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.|
|Strain description||Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).|
|Colony maintenance||Heterozygote x Wild-type [or Crossing to C57BL/6NCrSlc]|
|References||Oncogene, 8, 3313-3322 (1993). 7504233|
|Examination Date / Room / Rack||2020/02/17Room:3-CRack:E|
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)
|Donor DNA||herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA|
|Research application||Cancer Research|
Cell Biology Research
Immunology and Inflammation Research
Mouse Models for Human Disease
|Specific Term and Conditions||In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 8, 3313-3322 (1993).Please inform Laboratory for Animal Resources and Genetic Engineering,|
CDB (e mail: email@example.com) about the paper when published.
|Depositor||Ohtsura Niwa (Kyoto University)|
|Strain Status||Live mice|
|Strain Availability||Cryopreserved sperm (within 1 month)|
Live mouse (1 to 3 months)
|Additional Info.||Genotyping protocol -PCR-|
Mouse of the Month Jun 2005
BRC mice in Publications
Saito Y, Onishi N, Takami H, Seishima R, Inoue H, Hirata Y, Kameyama K, Tsuchihashi K, Sugihara E, Uchino S, Ito K, Kawakubo H, Takeuchi H, Kitagawa Y, Saya H, Nagano O.
Development of a functional thyroid model based on an organoid culture system.
Biochem. Biophys. Res. Commun. (2018) 29470983
Kobayashi M, Hoshino S, Abe T, Okita N, Tagawa R, Nagai W, Konno R, Suzuki Y, Furuya K, Ishikawa N, Okado H, Oku M, Iwamoto M, Miura Y, Sudo Y, Higami Y.
Identification of WWP1 as an obesity-associated E3 ubiquitin ligase with a protective role against oxidative stress in adipocytes.
Biochem. Biophys. Res. Commun. (2018) 30471861
Okita N, Ishikawa N, Mizunoe Y, Oku M, Nagai W, Suzuki Y, Matsushima S, Mikami K, Okado H, Sasaki T, Higami Y.
Inhibitory effect of p53 on mitochondrial content and function during adipogenesis.
Biochem. Biophys. Res. Commun. (2014) 24565844
Mai HN, Sharma G, Sharma N, Shin EJ, Kim DJ, Pham DT, Trinh QD, Jang CG, Nah SY, Jeong JH, Kim HC.
Genetic depletion of p53 attenuates cocaine-induced hepatotoxicity in mice.
Biochimie (2018) 30576773
Yamauchi H, Katayama K, Ueno M, Kanemitsu H, Nam C, Mikami T, Saito A, Ishida Y, Uetsuka K, Doi K, Ohmach Y, Nakayama H.
Etoposide induces TRP53-dependent apoptosis and TRP53-independent cell cycle arrest in trophoblasts of the developing mouse placenta.
Biol. Reprod. 80(4) 813-22(2009) 19109225
Sasaki H, Sugiyama M, Sasaki N.
Establishment of renal proximal tubule cell lines derived from the kidney of p53 knockout mice.
Cytotechnology (2019) 30603921
Tanikawa C, Zhang YZ, Yamamoto R, Tsuda Y, Tanaka M, Funauchi Y, Mori J, Imoto S, Yamaguchi R, Nakamura Y, Miyano S, Nakagawa H, Matsuda K.
The Transcriptional Landscape of p53 Signalling Pathway.
EBioMedicine 20 109-119(2017) 28558959
Tochitani T, Kanemitsu H, Yamauchi H, Uchida K, Nakayama H.
5-azacytidine, a chemotherapeutic drug, induces TRAIL-mediated apoptosis in mouse thymocytes in vivo.
Exp. Toxicol. Pathol. (2011) 20092982
Nam C, Doi K, Nakayama H.
Etoposide induces G2/M arrest and apoptosis in neural progenitor cells via DNA damage and an ATM/p53-related pathway.
Histol. Histopathol. 25(4) 485-93(2010) 20183801
Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Kotani N, Honke K, Suzumura A, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K.
Ganglioside GD3 enhances invasiveness of gliomas by forming a complex with platelet-derived growth factor receptor alpha and Yes.
J. Biol. Chem. (2015) 25940087
Tobisawa Y, Imai Y, Fukuda M, Kawashima H.
Sulfation of colonic mucins by N-acetylglucosamine 6-O-sulfotransferase-2 and its protective function in experimental colitis in mice.
J. Biol. Chem. 285(9) 6750-60(2010) 20018871
Okahashi N, Kohno S, Kitajima S, Matsuda F, Takahashi C, Shimizu H.
Metabolic characterization of cultured mammalian cells by mass balance analysis, tracer labeling experiments and computer-aided simulations.
J. Biosci. Bioeng. (2015) 25936961
Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues.
Meth. Enzymol. 479 243-56(2010) 20816170
Shin EJ, Nam Y, Lee JW, Nguyen PT, Yoo JE, Tran TV, Jeong JH, Jang CG, Oh YJ, Youdim MB, Lee PH, Nabeshima T, Kim HC.
N-Methyl, N-propynyl-2-phenylethylamine (MPPE), a Selegiline Analog, Attenuates MPTP-induced Dopaminergic Toxicity with Guaranteed Behavioral Safety: Involvement of Inhibitions of Mitochondrial Oxidative Burdens and p53 Gene-elicited Pro-apoptotic Change.
Mol. Neurobiol. (2015) 26563498
Kanemitsu H, Yamauchi H, Komatsu M, Yamamoto S, Okazaki S, Uchida K, Nakayama H.
6-mercaptopurine (6-MP) induces p53-mediated apoptosis of neural progenitor cells in the developing fetal rodent brain.
Neurotoxicol Teratol 31(4) 198-202(2009) 19281843
Kitajima S1, Kohno S, Kondoh A, Sasaki N, Nishimoto Y, Li F, Mohammed MS, Muranaka H, Nagatani N, Suzuki M, Kido Y, Takahashi C
Undifferentiated State Induced by Rb-P53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts
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