Strain Data Sheet
RBRC01351
Information1 | |
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| Image | |
| BRC No. | RBRC01351 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.129S-Grm2<tm1Nak>/NakRbrc |
| Former Common name | C57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO |
| H-2 Haplotype | |
| ES Cell line | CCE/EK.CCE [129S/SvEv-Gpi1<c>] |
| Background strain | |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Yokoi et al. (1996). A neomycin resistance cassette replaced 15.3-kb DNA fragment containing exon 2. The Grm2 deficient mice were produced by backcrossing to C57BL/6 strain for 20 times. |
| Strain description | mGluR2 (Grm2, G protein-coupled receptor, family C, group 1, member B) is widely dstributed both presynaptically and postsynaptically in varietry of neural cells. mGluR deficient mice have no behavioral abnormalities. But, a locomotor sensitization to cocaine administration was increased significantly. Moreover, significant impairment in motor coordination was detected. mGluR2 deficient mice were useful to clarify the rule in synaptic regulation of glutamatergic transmission in the neural network. |
| Colony maintenance | |
| References | Proc. Natl. Acad. Sci. USA, 102, 4170-4175 (1996). 15753323 Science, 273, 645-647 (1996). 8662555 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Grm2G protein-coupled receptor, family C, group 1, member B9Grm2<tm1Nak>targeted mutation 1, Shigetada Nakanishi4930441L02Rik, Grm2, mGluR2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Information2 | |
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| Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse glutamate receptor metabotropic 2 genomic DNA |
| Research application | Neurobiology Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT is required to obtain a prior written consent from the DEPOSITOR on use of the BIOLOGICAL RESOURCE. |
| Depositor | Shigetada Nakanishi (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Fribourg M, Moreno JL, Holloway T, Provasi D, Baki L, Mahajan R, Park G, Adney SK, Hatcher C, Eltit JM, Ruta JD, Albizu L, Li Z, Umali A, Shim J, Fabiato A, MacKerell AD Jr, Brezina V, Sealfon SC, Filizola M, González-Maeso J, Logothetis DE. Decoding the signaling of a GPCR heteromeric complex reveals a unifying mechanism of action of antipsychotic drugs. Cell 147(5) 1011-23(2011) 22118459 Moreno JL, Muguruza C, Umali A, Mortillo S, Holloway T, Pilar-Cuéllar F, Mocci G, Seto J, Callado LF, Neve RL, Milligan G, Sealfon SC, López-Giménez JF, Meana JJ, Benson DL, González-Maeso J. Identification of three residues essential for 5-hydroxytryptamine 2A-metabotropic glutamate 2 (5-HT2A·mGlu2) receptor heteromerization and its psychoactive behavioral function. J Biol Chem 287(53) 44301-19(2012) 23129762 Kim H, Lee Y, Park JY, Kim JE, Kim TK, Choi J, Lee JE, Lee EH, Kim D, Kim KS, Han PL. Loss of Adenylyl Cyclase Type-5 in the Dorsal Striatum Produces Autistic-Like Behaviors. Mol Neurobiol 54(10) 7994-8008(2017) 27878759 Moreno JL, Holloway T, Rayannavar V, Sealfon SC, González-Maeso J. Chronic treatment with LY341495 decreases 5-HT(2A) receptor binding and hallucinogenic effects of LSD in mice. Neurosci Lett 536 69-73(2013) 23333599 Moreno JL, Holloway T, Albizu L, Sealfon SC, González-Maeso J. Metabotropic glutamate mGlu2 receptor is necessary for the pharmacological and behavioral effects induced by hallucinogenic 5-HT2A receptor agonists. Neurosci Lett 493(3) 76-9(2011) 21276828 Moreno JL, Miranda-Azpiazu P, García-Bea A, Younkin J, Cui M, Kozlenkov A, Ben-Ezra A, Voloudakis G, Fakira AK, Baki L, Ge Y, Georgakopoulos A, Morón JA, Milligan G, López-Giménez JF, Robakis NK, Logothetis DE, Meana JJ, González-Maeso J. Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia. Sci Signal 9(410) ra5(2016) 26758213 |