RBRC01351

Information1

Image
BRC No.RBRC01351
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S-Grm2<tm1Nak>/NakRbrc
Former Common nameC57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Yokoi et al. (1996). A neomycin resistance cassette replaced 15.3-kb DNA fragment containing exon 2. The Grm2 deficient mice were produced by backcrossing to C57BL/6 strain for 20 times.
Strain descriptionmGluR2 (Grm2, G protein-coupled receptor, family C, group 1, member B) is widely dstributed both presynaptically and postsynaptically in varietry of neural cells. mGluR deficient mice have no behavioral abnormalities. But, a locomotor sensitization to cocaine administration was increased significantly. Moreover, significant impairment in motor coordination was detected. mGluR2 deficient mice were useful to clarify the rule in synaptic regulation of glutamatergic transmission in the neural network.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 102, 4170-4175 (1996). 15753323
Science, 273, 645-647 (1996). 8662555

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Grm2G protein-coupled receptor, family C, group 1, member B9Grm2<tm1Nak>targeted mutation 1, Shigetada Nakanishi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAmouse glutamate receptor metabotropic 2 genomic DNA, E. coli neo, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
Research applicationNeurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT is required to obtain a prior written consent from the DEPOSITOR on use of the BIOLOGICAL RESOURCE.
DepositorShigetada Nakanishi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Fribourg M, Moreno JL, Holloway T, Provasi D, Baki L, Mahajan R, Park G, Adney SK, Hatcher C, Eltit JM, Ruta JD, Albizu L, Li Z, Umali A, Shim J, Fabiato A, Mackerell AD Jr, Brezina V, Sealfon SC, Filizola M, Gonz lez-Maeso J, Logothetis DE.
Decoding the Signaling of a GPCR Heteromeric Complex Reveals a Unifying Mechanism of Action of Antipsychotic Drugs.
Cell 147(5) 1011-23(2011) 22118459

Moreno JL, Muguruza C, Umali A, Mortillo S, Holloway T, Pilar-Cu llar F, Mocci G, Seto J, Callado LF, Neve RL, Milligan G, Sealfon SC, L pez-Gim nez JF, Meana JJ, Benson DL, Gonz lez-Maeso J.
Identification of Three Residues Essential for 5-Hydroxytryptamine 2A-Metabotropic Glutamate 2 (5-HT2A{middle dot}mGlu2) Receptor Heteromerization and Its Psychoactive Behavioral Function.
J. Biol. Chem. 287(53) 44301-19(2012) 23129762

Kim H, Lee Y, Park JY, Kim JE, Kim TK, Choi J, Lee JE, Lee EH, Kim D, Kim KS, Han PL.
Loss of Adenylyl Cyclase Type-5 in the Dorsal Striatum Produces Autistic-Like Behaviors.
Mol. Neurobiol. (2016) 27878759

Moreno JL, Holloway T, Rayannavar V, Sealfon SC, Gonz lez-Maeso J.
Chronic treatment with LY341495 decreases 5-HT(2A) receptor binding and hallucinogenic effects of LSD in mice.
Neurosci. Lett. (2013) 23333599

Moreno JL, Holloway T, Albizu L, Sealfon SC, Gonz lez-Maeso J.
Metabotropic glutamate mGlu2 receptor is necessary for the pharmacological and behavioral effects induced by hallucinogenic 5-HT2A receptor agonists.
Neurosci. Lett. 493(3) 26542(2011) 21276828

Moreno JL, Miranda-Azpiazu P, García-Bea A, Younkin J, Cui M, Kozlenkov A, Ben-Ezra A, Voloudakis G, Fakira AK, Baki L, Ge Y, Georgakopoulos A, Morón JA, Milligan G, López-Giménez JF, Robakis NK, Logothetis DE, Meana JJ, González-Maeso J.
Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia.
Sci Signal 9(410) ra5. (2016) 26758213