Strain Data Sheet
RBRC01348
Information1 | |
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| Image | |
| BRC No. | RBRC01348 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | ICR.Cg-Trp53<tm1Sia>/Rbrc |
| Former Common name | ICR-p53+/- |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | albino [c/c] |
| Strain development | Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences. |
| Strain description | Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background). |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to Slc:ICR] |
| References | Oncogene, 8, 3313-3322 (1993). 7504233 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Information2 | |
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| Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA |
| Research application | Cancer Research Cell Biology Research Immunology and Inflammation Research Mouse Models for Human Disease |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 8, 3313-3322 (1993).Please inform Laboratory for Animal Resources and Genetic Engineering, CDB (e mail: mutant.bdr@riken.jp) about the paper when published. |
| Depositor | Ohtsura Niwa (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Mouse of the Month Jun 2005 Breeding characters Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Okuda A, Kurokawa S, Takehashi M, Maeda A, Fukuda K, Kubo Y, Nogusa H, Takatani-Nakase T, Okuda S, Ueda K, Tanaka S. Poly(ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells. BMC Neurosci 18(1) 14(2017) 28095779 Maruzuru Y, Koyanagi N, Takemura N, Uematsu S, Matsubara D, Suzuki Y, Arii J, Kato A, Kawaguchi Y. p53 Is a Host Cell Regulator during Herpes Simplex Encephalitis. J Virol 90(15) 6738-6745(2016) 27170756 |