Strain Data Sheet


Strain Information

BRC No.RBRC01346
TypeSpontaneous Mutation Congenic
SpeciesMus musculus
Strain nameC57BL/6-Prkdc<scid>/Rbrc
Former Common nameB6-scid, C57BL/6-scid mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack (a/a B/B C/C)
Strain developmentDeveloped by Mie University Graduate School of Medicine. The original scid mice were provided by Ohtura Niwa, Radiation Biology Center, Kyoto University (C57BL/6-scid p<un>). The mutant mice were backcrossed to DBA/2Cr and C57BL/6NCr (RBRC01300 and RBRC01346, respectively).
Strain descriptionscid, severe combined immune deficiency mice. Homozygous mutant mice lack both functional T and B cells, resulting in severe combined immunodeficiency. DBA/2 background (RBRC01300), C57BL/6 background (RBRC01346). We do not carry out a detection of leaky mice by measuring serum Ig level (PCR genotying of scid mutation only).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
ReferencesRadiat. Res., 157, 661-667(2002). 12005545

Health Report

Examination Date / Room / Rack2024/05/07Room:2-検Rack:F


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prkdcprotein kinase, DNA activated, catalytic polypeptide16Prkdc<scid>severe combined immune deficiency

Ordering Information

Donor DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for basic research, shall not use for commercial use.
DepositorKagemasa Kuribayashi (Mie University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Nagasaki J, Togashi Y, Sugawara T, Itami M, Yamauchi N, Yuda J, Sugano M, Ohara Y, Minami Y, Nakamae H, Hino M, Takeuchi M, Nishikawa H.
The critical role of CD4+ T cells in PD-1 blockade against MHC-II-expressing tumors such as classic Hodgkin lymphoma.
Blood Adv 4(17) 4069-4082(2020) 32870971

Ishino T, Kawashima S, Tanji E, Ueno T, Ueda Y, Ogasawara S, Sato K, Mano H, Ishihara S, Kato N, Kawazu M, Togashi Y.
Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens.
Br J Cancer (2023) 36732592

Kawase K, Kawashima S, Nagasaki J, Inozume T, Tanji E, Kawazu M, Hanazawa T, Togashi Y.
High Expression of MHC Class I Overcomes Cancer Immunotherapy Resistance Due to IFNγ Signaling Pathway Defects.
Cancer Immunol Res OF1-OF14(2023) 37062030

Kemmotsu N, Zhu L, Nagasaki J, Otani Y, Ueda Y, Dansako H, Fang Y, Date I, Togashi Y.
Combination therapy with hydrogen peroxide and irradiation promotes an abscopal effect in mouse models.
Cancer Sci (2023) 37485636

Zhou W, Kawashima S, Ishino T, Kawase K, Ueda Y, Yamashita K, Watanabe T, Kawazu M, Dansako H, Suzuki Y, Nishikawa H, Inozume T, Nagasaki J, Togashi Y.
Stem-like progenitor and terminally differentiated TFH-like CD4+ T cell exhaustion in the tumor microenvironment.
Cell Rep 43(2) 113797(2024) 38363680