Strain Data Sheet
RBRC01346
Information1 | |
|---|---|
| Image | |
| BRC No. | RBRC01346 |
| Type | Spontaneous Mutation Congenic |
| Species | Mus musculus |
| Strain name | C57BL/6-Prkdc<scid>/Rbrc |
| Former Common name | B6-scid, C57BL/6-scid mice |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6NCrSlc |
| Appearance | black (a/a B/B C/C) |
| Strain development | Developed by Mie University Graduate School of Medicine. The original scid mice were provided by Ohtura Niwa, Radiation Biology Center, Kyoto University (C57BL/6-scid p<un>). The mutant mice were backcrossed to DBA/2Cr and C57BL/6NCr (RBRC01300 and RBRC01346, respectively). |
| Strain description | scid, severe combined immune deficiency mice. Homozygous mutant mice lack both functional T and B cells, resulting in severe combined immunodeficiency. DBA/2 background (RBRC01300), C57BL/6 background (RBRC01346). We do not carry out a detection of leaky mice by measuring serum Ig level (PCR genotying of scid mutation only). |
| Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc] |
| References | Radiat. Res., 157, 661-667(2002). 12005545 |
Health Report | |
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| Examination Date / Room / Rack | 2023/08/07Room:2-検Rack:F 2023/05/08Room:2-検Rack:F 2023/02/06Room:2-検Rack:F 2022/11/07Room:2-検Rack:F 2022/08/08Room:2-検Rack:F 2022/05/09Room:2-検Rack:F |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Prkdcprotein kinase, DNA activated, catalytic polypeptide16Prkdc<scid>severe combined immune deficiency |
Information2 | |
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| Donor DNA | |
| Research application | Immunology and Inflammation Research |
| Specific Term and Conditions | The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for basic research, shall not use for commercial use. |
| Depositor | Kagemasa Kuribayashi (Mie University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Nagasaki J, Togashi Y, Sugawara T, Itami M, Yamauchi N, Yuda J, Sugano M, Ohara Y, Minami Y, Nakamae H, Hino M, Takeuchi M, Nishikawa H. The critical role of CD4+ T cells in PD-1 blockade against MHC-II-expressing tumors such as classic Hodgkin lymphoma. Blood Adv 4(17) 4069-4082(2020) 32870971 |