Strain Data Sheet



BRC No.RBRC01345
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129P2-Emx1<tm1.1(cre)Ito>/ItoRbrc
Former Common nameEmx1-Cre KI delta neo
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
AppearanceBlack [a/a B/B C/C]
Strain developmentDeveloped by Takuji Iwasato and Shigeyoshi Itohara, RIKEN Brain Science Institute. E14 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6J.
Strain descriptionEmx1-cre knock-in mice. neo type (inserted a cre recombinase gene and a frt flanked PGK-neo cassette into Emx1 gene)(RBRC00808), delta neo type (removed the pgk- neo cassette by FLP mediated recombination)(RBRC01345). The expression of cre recombinase was observed in dorsal telencephalon, neocortex, hippocampus, and olfactory bulb. In the delta neo type (RBRC01345), no ectopic cre expression was detected. Homozygous mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesNature, 406, 726-731 (2000). 10963597
Genesis, 38, 130-138 (2004). 15048810

Health Report

Examination Date / Room / Rack2022/04/11Room:3-ARack:L


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Emx1empty spiracles homolog 1 (Drosophila)6Emx1<tm1.1(cre)Ito>targeted mutation 1.1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase6cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6


Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) site, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 38, 130-138 (2004).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month June 2006
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, Takamatsu N, Yoshioka K.
JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration.
Cell Death Differ 22(8) 1260-74(2015) 25571974

Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.
Simultaneous detection of multiple transgenes for genetically-modified mouse strains.
Exp Anim 58(4) 437-42(2009) 19654444

Sugimoto J, Tanaka M, Sugiyama K, Ito Y, Aizawa H, Soma M, Shimizu T, Mitani A, Tanaka K.
Region-specific deletions of the glutamate transporter GLT1 differentially affect seizure activity and neurodegeneration in mice.
Glia 66(4) 777-788(2018) 29214672

Takagi T, Nishizaki Y, Matsui F, Wakamatsu N, Higashi Y.
De novo inbred heterozygous Zeb2/Sip1 mutant mice uniquely generated by germ-line conditional knockout exhibit craniofacial, callosal and behavioral defects associated with Mowat-Wilson syndrome.
Hum Mol Genet 24(22) 6390-402(2015) 26319231

Nagashima S, Ito N, Kobayashi R, Shiiba I, Shimura H, Fukuda T, Hagihara H, Miyakawa T, Inatome R, Yanagi S.
Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice.
J Biol Chem 100620(2021) 33811862

Mizusawa A, Watanabe A, Yamada M, Kamei R, Shimomura Y, Kitaura Y.
BDK Deficiency in Cerebral Cortex Neurons Causes Neurological Abnormalities and Affects Endurance Capacity.
Nutrients 12(8) (2020) 32751134