RBRC01308

Information1

Image
BRC No.RBRC01308
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hbegf<tm1Mek>/MekRbrc
Former Common nameHB-EGF lox Knock-in mice(BL6) (HBlox#80/B6)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Eisuke Mekada, Osaka University in 2003.
Strain descriptionB6.Cg-Hbegf<tm1Mek>. Hbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. Hbegf binds to and activates EGF receptor. The targeting vector containing WT HB-EGF cDNA flanked by loxP sites linked to lacZ reporter gene was transfered into TT2 ES cells.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJmsSlc)
ReferencesProc. Natl. Acad. Sci. USA, 100, 3221-3226 (2003). 12621152

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hbegfheparin-binding epidermal growth factor-like growth factor18Hbegf<tm1Mek>targeted mutation 1, Eisuke Mekada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hbegfheparin-binding epidermal growth factor-like growth factor (mouse)18Hbegf

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, mouse heparin-binding epidermal growth factor-like growth factor cDNA, poly A, E. coli LacZ, poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse heparin-binding epidermal growth factor-like growth factor genomic DNA
Research applicationCardiovascular Research
Cell Biology Research
Cre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
DepositorEisuke Mekada (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Apr 2006

BRC mice in Publications

Sasaki K, Omotuyi OI, Ueda M, Shinohara K, Ueda H.
NMDA receptor agonists reverse impaired psychomotor and cognitive functions associated with hippocampal Hbegf-deficiency in mice.
Mol Brain (2015) 26637193