BRC No.RBRC01307
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Hbegf<tm1.1Mek>/MekRbrc
Former Common nameHB-EGF Knockout mice(BALB) (HBdel#80/BALB)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cCrSlc
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Drs Ryo Iwamoto, Eisuke Mekada et al., at Osaka University in 2003.The HB-EGF null mice were generated by crossing with CAG-Cre transgenic mice.
Strain descriptionHbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. This protein binds to and activates the EGF receptor. Homozygous mutant mice exhibit grossly enlarged cardiac valves and ventricular chambers. Most homozygous mutant mice die by 3 weeks of age. LacZ gene is expressed under the native HB-EGF promoter to examine the expression pattern of the HB-EGF histologically.
Colony maintenanceBackcross to BALB/c (Heterozygote x BALB/cCrSlc)Most homozygous mutant mice were die by 3 weeks of age.
ReferencesProc. Natl. Acad. Sci. USA, 100, 3221-3226 (2003). 12621152

Health Report

Examination Date / Room / Rack2006/11/13Room:4-BRack:A


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hbegfheparin-binding epidermal growth factor-like growth factor18Hbegf<tm1.1Mek>targeted mutation 1.1, Eisuke Mekada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)18

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAPhage P1 loxP site, E. coli lacZ, poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse heparin-binding epidermal growth factor-like growth factor genomic DNA
Research applicationCardiovascular Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
DepositorEisuke Mekada (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Apr 2006

BRC mice in Publications

No Data